Year 2020, Volume 4 , Issue 9, Pages 771 - 774 2020-09-01

Gebelik sırasında nazal mukozada NFκb ve MUC5AC ekspresyon düzeylerinin değişimi
Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy

Burak ÜLKÜMEN [1] , Burcu ARTUNC-ULKUMEN [2] , Muhammet Burak BATİR [3] , Sırrı ÇAM [4]


Amaç: Üst solunum yolunun kronik enflamatuar hastalıklarının gebelikteki seyri konusunda çok sınırlı bilgi mevcuttur. Bu çalışmada, NFκb ve MUC5AC ile ilgili olarak hamilelik sırasında nazal mukozadaki biyomoleküler değişiklikleri ortaya çıkarmaya çalıştık. Bu sayede, gebeliğe bağlı burun rahatsızlıkları için yeni tedavi stratejileri önerme fırsatımız olabilir. Yöntemler: Sıçanlar üzerinde yapılan bu çalışma, Deney Hayvanları Araştırma ve Uygulama Merkezinde yapılmıştır. Otuz yetişkin Wister albino dişi sıçan, bir kontrol grubu ve bir hamile grubu olarak ayrıldı. Serum estradiole (E2) ve progesteron (PG) düzeyleri ELISA ile değerlendirildi. NFκb ve MUC5AC'nin nazal ekspresyonu, polimeraz zincir reaksiyon testi ile analiz edildi. İstatistiksel analiz, NFkb ve MUC5AC ekspresyonunun grup bazında karşılaştırılması ile yapıldı. Ayrıca E2 ve PG seviyelerinin bu moleküller üzerindeki etkisi de araştırıldı. Bulgular: Gebe sıçanlarda NFκb anlamlı olarak düşük (P=0,015) bulunurken MUC5AC anlamlı derecede yüksek bulundu (P=0.029). Serum PG ve NF-κB ekspresyonu arasında istatistiksel olarak anlamlı negatif korelasyon (P=0,027), progesteron ile MUC5AC ekspresyonu arasında ise pozitif korelasyon (P=0,017) bulundu. Ek olarak, estradiol ve MUC5AC arasında pozitif korelasyon tespit ettik (P=0.017). Sonuçlar: Bu çalışmada, sıçan nazal mukozasında NFκb ve MUC5AC'nin fizyolojik ekspresyonu ilk kez PCR ile gösterilmiştir. E’ ve PG’nin bu biyomoleküllerin ekspresyonu üzerindeki etkisi de gebelik bağlamında ortaya konmuştur. Bu bulgular, üst solunum yolunun gestasyonel enflamatuar değişikliklerini açıklama potansiyeline sahiptir.
Aim: There is very limited information concerning gestational course of chronic inflammatory diseases of the upper airway. In the current study, we tried to reveal biomolecular changes in the nasal mucosa during pregnancy regarding NFκb and MUC5AC. This way, we may have the opportunity to suggest new treatment strategies for pregnancy induced nasal disorders. Methods: Thirty adult female rats were separated into pregnant and control groups. Serum estradiole (E2) and progesterone (PG) levels were evaluated by ELISA. Nasal expression of NFκb and MUC5AC were analyzed by polymerase chain reaction testing. Relative expression of NFκb and MUC5AC was compared between two groups. In addition, the influences of PG and E2 levels on NFκb and MUC5AC were determined. Results: NFκb was significantly low (P=0.015), while MUC5AC was significantly high in pregnant rats (P=0.029). Serum PG had a significantly negative effect on NF-κB expression (P=0.027) and a significantly positive effect on MUC5AC expression (P=0.017). There was a positive correlation between estradiol and MUC5AC (P=0.017). Conclusions: In the current study, the physiological expression of NFκb and MUC5AC was shown for the first time by PCR in rat nasal mucosa. The effect of PG and E2 on the expression of these biomolecules in the context of pregnancy was also revealed. These findings have the potential to elucidate gestational inflammatory alterations in the upper airway.
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Primary Language en
Subjects Otorhinolaryngology
Journal Section Research article
Authors

Orcid: 0000-0003-1981-5886
Author: Burak ÜLKÜMEN (Primary Author)
Institution: manisa celal bayar üniversitesi
Country: Turkey


Orcid: 0000-0002-3128-8751
Author: Burcu ARTUNC-ULKUMEN
Institution: MANISA CELAL BAYAR UNIVERSITY
Country: Turkey


Orcid: 0000-0002-8722-5055
Author: Muhammet Burak BATİR
Institution: MANISA CELAL BAYAR UNIVERSITY
Country: Turkey


Orcid: 0000-0002-0972-8896
Author: Sırrı ÇAM
Institution: MANISA CELAL BAYAR UNIVERSITY
Country: Turkey


Supporting Institution Manisa Celal Bayar Üniversitesi
Project Number 2014-131
Dates

Publication Date : September 1, 2020

Bibtex @research article { josam789704, journal = {Journal of Surgery and Medicine}, issn = {}, eissn = {2602-2079}, address = {jsurgmed@gmail.com}, publisher = {Fatih BAŞAK}, year = {2020}, volume = {4}, pages = {771 - 774}, doi = {10.28982/josam.789704}, title = {Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy}, key = {cite}, author = {Ülkümen, Burak and Artunc-ulkumen, Burcu and Bati̇r, Muhammet Burak and Çam, Sırrı} }
APA Ülkümen, B , Artunc-ulkumen, B , Bati̇r, M , Çam, S . (2020). Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy . Journal of Surgery and Medicine , 4 (9) , 771-774 . DOI: 10.28982/josam.789704
MLA Ülkümen, B , Artunc-ulkumen, B , Bati̇r, M , Çam, S . "Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy" . Journal of Surgery and Medicine 4 (2020 ): 771-774 <http://jsurgmed.com/en/pub/issue/56766/789704>
Chicago Ülkümen, B , Artunc-ulkumen, B , Bati̇r, M , Çam, S . "Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy". Journal of Surgery and Medicine 4 (2020 ): 771-774
RIS TY - JOUR T1 - Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy AU - Burak Ülkümen , Burcu Artunc-ulkumen , Muhammet Burak Bati̇r , Sırrı Çam Y1 - 2020 PY - 2020 N1 - doi: 10.28982/josam.789704 DO - 10.28982/josam.789704 T2 - Journal of Surgery and Medicine JF - Journal JO - JOR SP - 771 EP - 774 VL - 4 IS - 9 SN - -2602-2079 M3 - doi: 10.28982/josam.789704 UR - https://doi.org/10.28982/josam.789704 Y2 - 2020 ER -
EndNote %0 Journal of Surgery and Medicine Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy %A Burak Ülkümen , Burcu Artunc-ulkumen , Muhammet Burak Bati̇r , Sırrı Çam %T Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy %D 2020 %J Journal of Surgery and Medicine %P -2602-2079 %V 4 %N 9 %R doi: 10.28982/josam.789704 %U 10.28982/josam.789704
ISNAD Ülkümen, Burak , Artunc-ulkumen, Burcu , Bati̇r, Muhammet Burak , Çam, Sırrı . "Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy". Journal of Surgery and Medicine 4 / 9 (September 2020): 771-774 . https://doi.org/10.28982/josam.789704
AMA Ülkümen B , Artunc-ulkumen B , Bati̇r M , Çam S . Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy. J Surg Med. 2020; 4(9): 771-774.
Vancouver Ülkümen B , Artunc-ulkumen B , Bati̇r M , Çam S . Change in expression of NFκb and MUC5AC in nasal mucosa during pregnancy. Journal of Surgery and Medicine. 2020; 4(9): 771-774.