Year 2018, Volume 2 , Issue 3, Pages 215 - 217 2018-09-01

Metisiline dirençli Staphylococcus aureus'u tespit etmek için bir tanı aracı olarak multipleks PCR kullanılması
Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus

Sadık AKGÜN [1] , Hakan Sezgin SAYİNER [2]


Amaç: Bu çalışmanın amacı, yoğun bakım ünitesi (YBÜ) hastalarında izole edilen, metisiline dirençli Staphylococcus aureus (MRSA) için tanısal test ve temas izolasyon stratejilerine ek olarak etkinliği araştırarak, MRSA raporlarını klinisyene daha hızlı bir şekilde iletmek ve tedavideki gecikmeleri önlemekti.

Yöntemler: Çalışmaya, 2015 ve 2017 yılları arasında, hastaneye yatırılmış YBÜ hastalarından alınan kültür örneklerinden izole edilen ve koagülaz pozitif olarak tanımlanan 320 Staphylococcus aureus suşu dahil edildi. Yara sürüntüleri alındı ve bakteri kültür işlemleri yapılarak, antibiyotik duyarlılık testi ve identifikasyon işlemleri için değerlendirildi. Swablardan alınan bu kültürler arasında MRSA olarak tanımlanan suşlar daha sonra hızlı Multipleks polimeraz zincir reaksiyonu (PCR) kullanılarak MecA geni için tarandı.

Bulgular: 320 suşun 67'sinde, tam otomatik kültür antibiyogram cihazı ile çalışılarak oksasilin direnci saptanarak MRSA tesbit edildi, Ayrıca hızlı Multipleks polimeraz zincir reaksiyonu (PCR) ile çalışıldığında bu 67 suşun 56'sında MecA geni tesbit edilerek, MRSA pozitifliği saptandı.

Sonuç: Multiplex PCR kullanarak hızlı tarama analizine bağlı olarak, % 86'dan fazla duyarlılık ile hastalar MRSA için erken tedavi alabildiler. Bu yöntem, bir tanı aracı olarak, diğer hastalıklarda da yararlı olabilir.

Aim: The goal of this report was to deliver the methicillin resistant Staphylococcus aureus (MRSA) reports to the clinician and prevent the treatment delays, investigating the efficacy in addition to diagnostic testing and contact isolation strategies for intensive care unit (ICU) patients with MRSA.

Methods: In this report, 320 Staphylococcus aureus strains identified as coagulase positive were cultured from hospitalized ICU patients between 2015 and 2017. Wound swabs were performed and bacteria cultures were evaluated for identification and antibiotic susceptibility testing using a culture antibiogram. Among these cultures from the swabs, MRSA was identified and subsequently screened for the MecA gene using rapid Multiplex polymerase chain reaction (PCR).

Results: MRSA was detected in 67 of 320 strains, because of oxacillin resistance was detected by working with a fully automated culture antibiogram device. In addition, MRSA positivity was detected because of the high MecA gene expression in 56 of these 67 strains using rapid multiplex PCR. 

Conclusion: With greater than 86% sensitivity, patients were able to get early treatment for MRSA due to the rapid screening analysis using Multiplex PCR. This method, as a diagnostic tool, may be of benefit in other diseases.

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Primary Language en
Subjects Medicine
Journal Section Research article
Authors

Author: Sadık AKGÜN (Primary Author)
Institution: Adiyaman University, Training and Research Hospital, Adiyaman, Türkiye
Country: Turkey


Author: Hakan Sezgin SAYİNER
Institution: Adiyaman University, Training and Research Hospital, Adiyaman, Türkiye
Country: Turkey


Dates

Publication Date : September 1, 2018

Bibtex @research article { josam415215, journal = {Journal of Surgery and Medicine}, issn = {}, eissn = {2602-2079}, address = {jsurgmed@gmail.com}, publisher = {Fatih BAŞAK}, year = {2018}, volume = {2}, pages = {215 - 217}, doi = {10.28982/josam.415215}, title = {Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus}, key = {cite}, author = {Akgün, Sadık and Sayiner, Hakan Sezgin} }
APA Akgün, S , Sayiner, H . (2018). Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus . Journal of Surgery and Medicine , 2 (3) , 215-217 . DOI: 10.28982/josam.415215
MLA Akgün, S , Sayiner, H . "Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus" . Journal of Surgery and Medicine 2 (2018 ): 215-217 <https://jsurgmed.com/en/pub/issue/36726/415215>
Chicago Akgün, S , Sayiner, H . "Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus". Journal of Surgery and Medicine 2 (2018 ): 215-217
RIS TY - JOUR T1 - Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus AU - Sadık Akgün , Hakan Sezgin Sayiner Y1 - 2018 PY - 2018 N1 - doi: 10.28982/josam.415215 DO - 10.28982/josam.415215 T2 - Journal of Surgery and Medicine JF - Journal JO - JOR SP - 215 EP - 217 VL - 2 IS - 3 SN - -2602-2079 M3 - doi: 10.28982/josam.415215 UR - https://doi.org/10.28982/josam.415215 Y2 - 2018 ER -
EndNote %0 Journal of Surgery and Medicine Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus %A Sadık Akgün , Hakan Sezgin Sayiner %T Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus %D 2018 %J Journal of Surgery and Medicine %P -2602-2079 %V 2 %N 3 %R doi: 10.28982/josam.415215 %U 10.28982/josam.415215
ISNAD Akgün, Sadık , Sayiner, Hakan Sezgin . "Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus". Journal of Surgery and Medicine 2 / 3 (September 2018): 215-217 . https://doi.org/10.28982/josam.415215
AMA Akgün S , Sayiner H . Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus. J Surg Med. 2018; 2(3): 215-217.
Vancouver Akgün S , Sayiner H . Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus. Journal of Surgery and Medicine. 2018; 2(3): 215-217.
IEEE S. Akgün and H. Sayiner , "Using multiplex PCR as a diagnostic tool to detect methicillin resistant Staphylococcus aureus", Journal of Surgery and Medicine, vol. 2, no. 3, pp. 215-217, Sep. 2018, doi:10.28982/josam.415215