Year 2020, Volume 4 , Issue 5, Pages 346 - 350 2020-05-01

Propolisin anksiyeteye ve bazı biyokimyasal parametrelere etkilerinin sempatik deri cevabı ve yükseltilmiş T labirent ile araştırılması
Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze

Mustafa NİSARİ [1] , Memet Hanifi EMRE [2] , Nazan DOLU [3] , Hale ACER [4] , Ferhat PEKTAŞ [5]


Amaç: Propolisin anti-mikrobiyal, antioksidan, anti-tümör, anksiyolitik ve anti-inflamatuar etkilere sahip olduğu gösterilmiştir. Ancak literatürde propolisin anksiyojenik etkisi konusundaki çalışmalara rastlanılmamıştır. Bu çalışmamızda, soğuk stresi oluşturulmuş sıçanlarda propolisin farklı dozlarının anksiyeteye etkilerinin sempatik deri cevabı (elektrodermal aktivite) ve yükseltilmiş T labirent ile araştırılması amaçlandı.
Yöntemler: Çalışmada 40 adet Wistar albino erkek sıçan kullanıldı. Kontrol grubu, düşük doz (10 mg/kg PRO), orta doz (30 mg/kg PRO) ve yüksek doz propolis (50 mg/kg PRO) grupları oluşturularak, kontrol grubu hariç diğer gruplara propolis gavaj yoluyla uygulandı. Enjeksiyondan 20 dakika sonra, sıçanların anksiyete skorları yükseltilmiş T-labirent ile değerlendirildi ve daha sonra da elektrodermal aktiviteleri (EDA) ölçüldü. Deney sonunda kan örneklerinde, spektrofotometrik olarak malondialdehit (MDA) ile bazı enzimatik ve lipid değerleri ölçüldü.
Bulgular: 10 mg/kg PRO grubunda açık kolda harcanan zamanın yüzdesi ve açık kola giriş sayısı, diğer gruplara göre daha düşük bulunurken 30 mg/kg PRO grubunda artış gözlendi. EDA, 30 mg/kg PRO (sırasıyla, P=0,012, P=0,02) ve 50 mg/kg PRO (sırasıyla, P=0,013, P=0,02 gruplarında kontrole kıyasla daha düşüktü. MDA’nın 30 mg/kg PRO ve 50 mg/kg PRO gruplarında önemli oranda düşük olduğu görüldü. 30 mg/kg PRO grubunda AST değeri artış gösterirken ALT değerinde azalmanın olduğu görüldü. Toplam kolesterol ve trigliserit değerleri 50 mg/kg PRO grubunda anlamlı olarak düşüktü. HDL değerinin düşük doz propolisten uygulamasından itibaren anlamlı yükseldiği, LDL değerinin ise sadece 10 mg/kg PRO grubunda anlamlı azaldığı olduğu görüldü.
Sonuçlar: EDA ve T-labirent yöntemleriyle elde edilen sonuçlara göre düşük ve orta doz propolis anksiyetik etki yaparken, yüksek doz propolis anksiyolitik etki göstermiştir. Ayrıca sıçanlara soğuk stres sonucunda uygulanan propolisin MDA değerlerini orta ve yüksek doz gruplarında düşürdüğü, enzimatik ve lipid değerlerinde ise organizma lehine olumlu değişimlere sebep olduğu görüldü. Dolayısıyla propolisin anksiyeteye ilişkin yanıtlarının, doza bağlı etkilerine göre değiştiği sonucuna varılmıştır.

Aim: Propolis has been shown to have anti-microbial, antioxidant, anti-tumor, anxiolytic and anti-inflammatory effects. However, to the best of our knowledge, there are no studies on its anxiogenic effects. In this study, we aimed to investigate the effects of different doses of propolis on anxiety in rats with cold stress via sympathetic skin response (electrodermal activity) and elevated T maze.
Methods: Forty Wistar albino male rats were used in the study, divided into four groups: The control group, low dose (10 mg/kg PRO), medium dose (30 mg/kg PRO) and high dose propolis groups (50 mg/kg PRO). Propolis was administered via gavage to all rats except the control group. Twenty minutes after injection, the anxiety scores of the rats were evaluated with an elevated T-maze, and their electrodermal activities (EDA) were measured. At the end of the experiment, some enzymatic and lipid values were measured with malondialdehyde (MDA) in blood samples.
Results: The percentage of time spent on open arms and the number of open arm entries were lower in the 10mg/kg PRO group, while an increase was observed in the 30 mg/kg PRO group. EDA values were lower in the 30 mg / kg PRO (P=0.012; P=0.02, respectively) and 50 mg / kg PRO (P=0.013, P=0.02, respectively) groups as compared to the control group. MDA was significantly lower in the 30 mg/kg PRO and 50 mg/kg PRO groups. While AST value increased in the 30 mg / kg PRO group, ALT value decreased. Total cholesterol and triglyceride values were significantly lower in the 50 mg / kg PRO group. HDL value increased significantly after administration of propolis and LDL value decreased significantly only in the 10 mg/kg PRO group.
Conclusions: According to the results obtained by EDA and T labyrinth methods, while low and high dose propolis, which was administered to rats after cold stress, showed an anxiogenic effect, medium dose propolis exerted an anxiolytic effect. It also decreased MDA values in the medium and high dose groups and influenced enzymatic and lipid values in favor of the rat. It was concluded that the anxiety-related effects of propolis were dose-dependent.
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Primary Language en
Subjects Biochemistry and Molecular Biology
Journal Section Research article
Authors

Orcid: 0000-0001-7469-8921
Author: Mustafa NİSARİ (Primary Author)
Institution: NUH NACI YAZGAN UNIVERSITY
Country: Turkey


Orcid: 0000-0001-8695-5913
Author: Memet Hanifi EMRE
Institution: BAHCESEHIR UNIVERSITY
Country: Turkey


Orcid: 0000-0002-3104-7587
Author: Nazan DOLU
Institution: Baskent University
Country: Turkey


Orcid: 0000-0002-7823-2034
Author: Hale ACER
Institution: Sağlık Bakanlığı Bursa Şehir Hastanesi
Country: Turkey


Orcid: 0000-0002-1862-9515
Author: Ferhat PEKTAŞ
Institution: AHI EVRAN UNIVERSITY
Country: Turkey


Supporting Institution İnönü Üniversitesi BAPKB
Project Number 2015/80 no’lu çalışma
Dates

Publication Date : May 1, 2020

Bibtex @research article { josam726017, journal = {Journal of Surgery and Medicine}, issn = {}, eissn = {2602-2079}, address = {jsurgmed@gmail.com}, publisher = {Fatih BAŞAK}, year = {2020}, volume = {4}, pages = {346 - 350}, doi = {10.28982/josam.726017}, title = {Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze}, key = {cite}, author = {Ni̇sari̇, Mustafa and Emre, Memet and Dolu, Nazan and Acer, Hale and Pektaş, Ferhat} }
APA Ni̇sari̇, M , Emre, M , Dolu, N , Acer, H , Pektaş, F . (2020). Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze. Journal of Surgery and Medicine , 4 (5) , 346-350 . DOI: 10.28982/josam.726017
MLA Ni̇sari̇, M , Emre, M , Dolu, N , Acer, H , Pektaş, F . "Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze". Journal of Surgery and Medicine 4 (2020 ): 346-350 <http://jsurgmed.com/en/pub/issue/54145/726017>
Chicago Ni̇sari̇, M , Emre, M , Dolu, N , Acer, H , Pektaş, F . "Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze". Journal of Surgery and Medicine 4 (2020 ): 346-350
RIS TY - JOUR T1 - Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze AU - Mustafa Ni̇sari̇ , Memet Emre , Nazan Dolu , Hale Acer , Ferhat Pektaş Y1 - 2020 PY - 2020 N1 - doi: 10.28982/josam.726017 DO - 10.28982/josam.726017 T2 - Journal of Surgery and Medicine JF - Journal JO - JOR SP - 346 EP - 350 VL - 4 IS - 5 SN - -2602-2079 M3 - doi: 10.28982/josam.726017 UR - https://doi.org/10.28982/josam.726017 Y2 - 2020 ER -
EndNote %0 Journal of Surgery and Medicine Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze %A Mustafa Ni̇sari̇ , Memet Emre , Nazan Dolu , Hale Acer , Ferhat Pektaş %T Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze %D 2020 %J Journal of Surgery and Medicine %P -2602-2079 %V 4 %N 5 %R doi: 10.28982/josam.726017 %U 10.28982/josam.726017
ISNAD Ni̇sari̇, Mustafa , Emre, Memet , Dolu, Nazan , Acer, Hale , Pektaş, Ferhat . "Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze". Journal of Surgery and Medicine 4 / 5 (May 2020): 346-350 . https://doi.org/10.28982/josam.726017
AMA Ni̇sari̇ M , Emre M , Dolu N , Acer H , Pektaş F . Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze. J Surg Med. 2020; 4(5): 346-350.
Vancouver Ni̇sari̇ M , Emre M , Dolu N , Acer H , Pektaş F . Investigation of dose related effects of propolis on anxiety and some biochemical parameters with sympathetic skin response and increased T-maze. Journal of Surgery and Medicine. 2020; 4(5): 350-346.