Does intratympanic Mesna application prevent cholesteatoma? An experimental study on rats

Bilal Sizer; Songül Demir; Aylin Gül

doi:10.28982/josam.974266

Authors

Bilal Sizer https://orcid.org/0000-0003-2604-5015
Songül Demir Mardin Devlet Hastanesi https://orcid.org/0000-0002-0616-7085
Aylin Gül Gaziantep Medikal Park Hastanesi https://orcid.org/0000-0003-3715-8744

DOI:

https://doi.org/10.28982/josam.974266

Keywords:

Mesna, cholesteatoma, Fibrosis, Otitis media, Intratympanic

Abstract

Background/Aim: Cholesteatoma is an invasive and destructive disease, responsible for most of the complications related to chronic otitis media. The only effective treatment is surgical excision. Researching medical treatment alternatives can bring a new perspective to the treatment of this disease. This study aimed to investigate the effect of Mesna on otitis media and cholesteatoma induced by propylene glycol on an experimental animal model. Methods: The study was designed to consist of sixteen Wistar albino rats, with their right ears being the control group and the left ears being the experiment group. Fifty percent propylene glycol, gentamicin sulfate, and physiologic saltwater were administered to the right ear, and 50% propylene glycol, gentamicin sulfate, and 20% Mesna were administered to the left ear through intratympanic injections on days 1, 3, 8, 15, and 21. The rats were sacrificed 45 days after the first injection and underwent histopathological examination. Results: Cholesteatoma and fibrosis were less common in the experimental group. In the study group, the average and maximum thicknesses of the tympanic membranes (P=0.008) and the minimum thicknesses of the tympanic bulla (P=0.019) were significantly less than those of the control group. Conclusion: In the experimental cholesteatoma model created in rats, Mesna, administered intratympanically, was seen to completely prevent the formation of cholesteatoma. However, histopathological examination revealed that although present, cholesteatoma formation and fibrosis were significantly less in the experimental group.

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