The effects of exposure to endocrine-disrupting chemicals in intrauterine life on thyroid function tests during the neonatal period



Bisphenol A, di-(2-ethylhexyl) phthalate, Mono-ethylhexyl phthalate, Cord blood, Thyroid function


Background/Aim: Animal studies have shown that endocrine-disrupting chemicals can cause transient hypothyroidism. The aim of this study is to investigate the effects of exposure to endocrine disrupting chemicals (polychlorinated biphenyls (PCBs), polybrominated biphenyls (PBBs), bisphenol A (BPA) in plastics) in intrauterine life on thyroid function tests during the neonatal period. Methods: In this observational cohort study, cord blood samples were obtained from all infants at birth to measure endocrine disruptors. Serum bisphenol A, di-(2-ethylhexyl) phthalate, and mono-ethylhexyl phthalate levels were measured by high performance liquid chromatography (HPLC). We excluded newborns whose mothers had thyroid function disorders. Results: The male newborns’ cord bisphenol A concentrations were significantly higher than those of female newborns (1.14 (0.26) ng/ml vs 0.85 (0.25) ng/ml, respectively; P=0.007). When we examined the correlation between the cord blood phthalate values and the maternal and newborn’s thyroid function tests, a negative relationship between mono-ethylhexyl phthalate and newborn thyroid stimulating hormone was detected (r= -0.284, P=0.003). Conclusion: A negative correlation was detected between cord blood mono-ethylhexyl phthalate levels and neonatal thyroid stimulating hormone levels suggesting that phthalate exposure may affect the thyroid function of babies in the prenatal period.


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Bigsby R, Chapin RE, Daston GP, Davis BJ, Gorski J, Gray LE, et al. Evaluating the effects of endocrine disruptors on endocrine function during development. Environ Health Perspect. 1999;107:613-8.

Lee MM. Endocrine Disrupters. A Current Review of Pediatric Endocrinology. 2007;109-18.

Kelce WR, Wilson EM. Antiandrogenic Effects of Environmental Endocrine Disruptors. In: Metzler M (ed). The Handbook of Environmental Chemistry, Endocrine Disruptors Part 1. Springer-Verlag Berlin Heidelberg; 2001. p. 39-61.

Romano ME, Webster GM, Vuong AM, Thomas Zoeller R, Chen A, Hoofnagle AN, et al. Gestational urinary bisphenol A and maternal and newborn thyroid hormone concentrations: the HOME Study. Environ Res. 2015 Apr;138:453-60.

Solomon GM, Schettler T. Environment and health. 6.Endocrine disruption and potential human health implications. CMAJ Canadian Medicael Asoociation Journal. 2000;1116:1467-74.

Ghassabian A, Bongers-Schokking JJ, Henrichs J, Jaddoe VW, Visser TJ, Visser W, et al. Maternal thyroid function during pregnancy and behavioral problems in the offspring: the generation R study. Pediatr Res. 2011 May;69:454–9.

Xu X, Liu Y, Sadamatsu M, Tsutsumi S, Akaike M, Ushijima H, Kato N. Perinatal bisphenol A affects the behavior and SRC-1 expression of male pups but does not influence on the thyroid hormone receptors and its responsive gene. Neurosci Res. 2007 Jun;58(2):149-55. doi: 10.1016/j.neures.2007.02.011. Epub 2007 Feb 23. PMID: 17412439.

Zoeller RT, Bansal R, Parris C. Bisphenol-A, an environmental contaminant that acts as a thyroid hormone receptor antagonist in vitro, increases serum thyroxine, and alters RC3/neurogranin expression in the developing rat brain. Endocrinology. 2005 Feb;146(2):607-12. doi: 10.1210/en.2004-1018. Epub 2004 Oct 21. PMID: 15498886.

Meeker JD, Calafat AM, Hauser R. Urinary bisphenol A concentrations in relation to serum thyroid and reproductive hormone levels in men from an infertility clinic. Environ Sci Technol. 2010 Feb 15;44(4):1458-63.

Buckıova D, Kyselova V, Pıknıcova J, Boubelık M. Low Doses of Bisphenol A Affect Fertility in CD Mice. Reprod Toxicol. 2001;15:459.

Richter CA, Birnbaum LS, Farabollini F, Newbold RR, Rubin BS, Talsness CE, et al. In vivo effects of bisphenol A in laboratory rodent studies. Reprod Toxicol. 2007:24:199-224.

Khurana S, Ranmal S, Ben Jonathan N. Exposure of newborn male and female rats to environmental estrogens: delayed and sustained hyperprolactinemia and alterations in estrogen receptor expression. Endocrinology. 2000;141:4512–7.

Dolinoy DC, Huang D, Jirtle RL. Maternal nutrient supplementation counteracts bisphenol A-induced DNA hypomethylation in early development. Proc Natl Acad Sci USA. 2007;104(32):13056-61.

Guida M, Troisi J, Ciccone C, Granozio G, Cosimato C. Bisphenol A and congenital developmental defects in humans. J Expo Sci Environ Epidemiol. 2015 Apr;774:33-9. doi: 10.1016/j.mrfmmm.2015.02.007.

Frederiksen H, Aksglaede L, Sorensen K, Skakkebaek NE, Juul A, Andersson AM. Urinary excretion of phthalate metabolites in 129 healthy Danish children and adolescents: estimation of daily phthalate intake. Environ Res. 2011 Jul;111(5):656-63. doi: 10.1016/j.envres.2011.03.005. Epub 2011 Mar 22. PMID: 21429484.

Chevrier J, Gunier RB, Bradman A, Holland NT, Calafat AM, Eskenazi B, Harley KG. Maternal urinary bisphenol a during pregnancy and maternal and neonatal thyroid function in the CHAMACOS study. Environ Health Perspect. 2013 Jan;121(1):138-44. doi: 10.1289/ehp.1205092. Epub 2012 Oct 4. PMID: 23052180; PMCID: PMC3553432.

Schönfelder G, Wittfoht W, Hopp H, Talsness CE, Paul M, Chahoud I. Parent bisphenol A accumulation in the human maternal-fetal-placental unit. Environ Health Perspect. 2002 Nov;110(11):A703-707.

Padmanabhan V, Siefert K, Ransom S, Johnson T, Pinkerton J, Anderson L, et al. Maternal bisphenol-A levels at delivery: a looming problem? J Perinatol. 2008;28:258–63.

Lee YJ, Ryu HY, Kim HK, Min CS, Lee JH, Kim E, Nam BH, Park JH, Jung JY, Jang DD, Park EY, Lee KH, Ma JY, Won HS, Im MW, Leem JH, Hong YC, Yoon HS. Maternal and fetal exposure to bisphenol A in Korea. Reprod Toxicol. 2008 Aug;25(4):413-9. doi: 10.1016/j.reprotox.2008.05.058. Epub 2008 May 25. PMID: 18577445.

Takeuchi T, Tsutsumi O. Serum bisphenol a concentrations showed gender differences, possibly linked to androgen levels. Biochem Biophys Res Commun. 2002 Feb 15;291(1):76-8. doi: 10.1006/bbrc.2002.6407. PMID: 11829464.

Whyatt RM, Liu X, Rauh VA, Calafat AM, Just AC, Hoepner L, Diaz D, Quinn J, Adibi J, Perera FP, Factor-Litvak P. Maternal prenatal urinary phthalate metabolite concentrations and child mental, psychomotor, and behavioral development at 3 years of age. Environ Health Perspect. 2012 Feb;120(2):290-5. doi: 10.1289/ehp.1103705. Epub 2011 Sep 6. PMID: 21893441; PMCID: PMC3279439.

Boas M, Frederiksen H, Feldt-Rasmussen U, Skakkebæk NE, Hegedüs L, Hilsted L, et al. Childhood exposure to phthalates: associations with thyroid function, insulin-like growth factor I, and growth. Environ Health Perspect. 2010 Oct;118(10):1458-64.

Wolff MS, Engel SM, Berkowitz GS, Ye X, Silva MJ, Zhu C, Wetmur J, Calafat AM. Prenatal phenol and phthalate exposures and birth outcomes. Environ Health Perspect. 2008 Aug;116(8):1092-7. doi: 10.1289/ehp.11007. PMID: 18709157; PMCID: PMC2516577.






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Özsoylu S, Güneş T, Akın L, Kendirci M. The effects of exposure to endocrine-disrupting chemicals in intrauterine life on thyroid function tests during the neonatal period. J Surg Med [Internet]. 2021 Jun. 1 [cited 2024 May 20];5(6):583-7. Available from: