Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency



Follicle count, Fragmentation, Ovarian insufficiency, Ovarian striation, Rats


Background/Aim: Primary ovarian insufficiency (POI) is defined as the depletion of the primordial follicle pool in women under the age of 40. New methods for stimulating ovarian follicle cells are being investigated in order to ensure the continuity of the menstrual cycle and fertility. The present study aimed to compare follicle reserves after ovarian striation or ovarian fragmentation in rats with ovarian insufficiency. Methods: Thirty adult female rats in the estrus phase were randomized into three groups. Group 1 and Group 2 were medicated with intraperitoneal 7.5 mg/kg paclitaxel to create ovarian insufficiency. Group 3 was the control group, and intraperitoneal 3 mL 0.9% sterile saline solution was administered. The first laparotomy was performed to evaluate ovarian insufficiency 1 week after chemotherapy. In Group 1, the right ovarian cortex was striated using an insulin injector. In Group 2, the right ovary was divided into five parts. These five pieces were transferred to the pocket created under the right pelvic peritoneum. In Group 3, only laparotomy was performed. After 1 month, all rats underwent a second laparotomy, and the number of ovarian follicles (primordial, primary, secondary, antral) were compared, as were their serum follicle-stimulating hormone (FSH) and estradiol (E2) levels. Results: There was a significant difference in the number of follicles among all three groups (P<0.05). The number of follicles (primordial, primary, secondary, antral) was significantly higher in the striated group than in the fragmented group (P<0.001). There were no statistically significant differences between the three groups in terms of mean serum FSH and E2 values measured at the second laparotomy (P>0.05). Conclusion: Ovarian striation on the ovary cortex may be a new method for the treatment of ovarian insufficiency.


Download data is not yet available.


Nelson LM. Clinical practice. Primary ovarian insufficiency. N Engl J Med. 2009 Feb 5;360(6):606-14. doi: 10.1056/NEJMcp0808697.

Kawamura K. IVA and ovarian tissue cryopreservation. In: Suzuki N, Donnez J, (eds). Gonadal tissue cryopreservation in fertility preservation. Japan: Springer, 2016;149-60. doi: 10.1007/978-4-431-55963-4_10.

European Society for Human Reproduction and Embryology (ESHRE) Guideline Group on POI, Webber L, Davies M, Anderson R, Bartlett J, Braat D, Cartwright B, et al. ESHRE Guideline: management of women with premature ovarian insufficiency. Hum Reprod. 2016 May;31(5):926-37. doi: 10.1093/humrep/dew027.

Golezar S, Ramezani Tehrani F, Khazaei S, Ebadi A, Keshavarz Z. The global prevalence of primary ovarian insufficiency and early menopause: a meta-analysis. Climacteric. 2019 Aug;22(4):403-11. doi: 10.1080/13697137.2019.1574738.

Bidet M, Bachelot A, Touraine P. Premature ovarian failure: predictability of intermittent ovarian function and response to ovulation induction agents. Curr Opin Obstet Gynecol. 2008 Aug;20(4):416-20. doi: 10.1097/GCO.0b013e328306a06b.

Parrot DMV. The fertility of mice with orthotopic ovarian grafts derived from frozen tissue. J Reprod Fertil. 1960; 1: 230-41.

Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, et al. Livebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet. 2004 Oct 16-22;364(9443):1405-10. doi: 10.1016/S0140-6736(04)17222-X.

Kawamura K, Cheng Y, Suzuki N, Deguchi M, Sato Y, Takae S, et al. Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment. Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17474-9. doi: 10.1073/pnas.1312830110.

Zhang X, Han T, Yan L, Jiao X, Qin Y, Chen ZJ. Resumption of Ovarian Function After Ovarian Biopsy/Scratch in Patients With Premature Ovarian Insufficiency. Reprod Sci. 2019 Feb;26(2):207-13. doi: 10.1177/1933719118818906.

Viana JH, Dorea MD, Siqueira LG, Arashiro EK, Camargo LS, Fernandes CA, et al. Occurrence and characteristics of residual follicles formed after transvaginal ultrasound-guided follicle aspiration in cattle. Theriogenology. 2013 Jan 15;79(2):267-73. doi: 10.1016/j.theriogenology.2012.08.015.

Yucebilgin MS, Terek MC, Ozsaran A, Akercan F, Zekioglu O, Isik E, et al. Effect of chemotherapy on primordial follicular reserve of rat: an animal model of premature ovarian failure and infertility. Aust N Z J Obstet Gynaecol. 2004 Feb;44(1):6-9. doi: 10.1111/j.1479-828X.2004.00143.x.

Myers M, Britt KL, Wreford NG, Ebling FJ, Kerr JB. Methods for quantifying follicular numbers within the mouse ovary. Reproduction. 2004 May;127(5):569-80. doi: 10.1530/rep.1.00095.

Decanter C, Cloquet M, Dassonneville A, D'Orazio E, Mailliez A, Pigny P. Different patterns of ovarian recovery after cancer treatment suggest various individual ovarian susceptibilities to chemotherapy. Reprod Biomed Online. 2018 Jun;36(6):711-8. doi: 10.1016/j.rbmo.2018.02.004.

Ben-Aharon I, Granot T, Meizner I, Hasky N, Tobar A, Rizel S, et al. Long-Term Follow-Up of Chemotherapy-Induced Ovarian Failure in Young Breast Cancer Patients: The Role of Vascular Toxicity. Oncologist. 2015 Sep;20(9):985-91. doi: 10.1634/theoncologist.2015-0044.

Wang TH, Wang HS, Soong YK. Paclitaxel-induced cell death: where the cell cycle and apoptosis come together. Cancer. 2000 Jun 1;88(11):2619-28. doi: 10.1002/1097-0142(20000601)88:11<2619::aid-cncr26>;2-j.

Gücer F, Balkanli-Kaplan P, Doganay L, Yüce MA, Demiralay E, Sayin NC, et al. Effect of paclitaxel on primordial follicular reserve in mice. Fertil Steril. 2001 Sep;76(3):628-9. doi: 10.1016/s0015-0282(01)01959-8.

Li J, Kawamura K, Cheng Y, Liu S, Klein C, Liu S, et al. Activation of dormant ovarian follicles to generate mature eggs. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10280-4. doi: 10.1073/pnas.1001198107.

Yildirim M, Noyan V, Bulent Tiras M, Yildiz A, Guner H. Ovarian wedge resection by minilaparatomy in infertile patients with polycystic ovarian syndrome: a new technique. Eur J Obstet Gynecol Reprod Biol. 2003 Mar 26;107(1):85-7. doi: 10.1016/s0301-2115(02)00348-2.

Debras E, Fernandez H, Neveu ME, Deffieux X, Capmas P. Ovarian drilling in polycystic ovary syndrome: Long term pregnancy rate. Eur J Obstet Gynecol Reprod Biol X. 2019 Aug 13;4:100093. doi: 10.1016/j.eurox.2019.100093.

Eftekhar M, Deghani Firoozabadi R, Khani P, Ziaei Bideh E, Forghani H. Effect of Laparoscopic Ovarian Drilling on Outcomes of In Vitro Fertilization in Clomiphene-Resistant Women with Polycystic Ovary Syndrome. Int J Fertil Steril. 2016 Apr-Jun;10(1):42-7. doi: 10.22074/ijfs.2016.4767.

Ginther OJ, Bergfelt DR, Kulick LJ, Kot K. Selection of the dominant follicle in cattle: establishment of follicle deviation in less than 8 hours through depression of FSH concentrations. Theriogenology. 1999 Oct 15;52(6):1079-93. doi: 10.1016/S0093-691X(99)00196-X.

Viana JHM, Ferreira AM, Camargo LSA, Sa WF, Araujo MCC, Fernandes CAC, et al. Effect of the presence of non-regressed follicles after oocyte pick-up on follicular dynamics of Bos indicus cattle. Abstracts for poster presentations. Theriogenology. 2001;55:534. doi:10.1016/S0093-691X(00)00457-X.

Stern CJ, Toledo MG, Hale LG, Gook DA, Edgar DH. The first Australian experience of heterotopic grafting of cryopreserved ovarian tissue: evidence of establishment of normal ovarian function. Aust N Z J Obstet Gynaecol. 2011 Jun;51(3):268-75. doi: 10.1111/j.1479-828X.2011.01289.x.

Zhang H, Yang Y, Ma W, Wu H, Zheng X, Hei C, et al. The revascularization and follicular survival of mouse ovarian grafts treated with FSH during cryopreservation by vitrification. Cryo Letters. 2016 Mar-Apr;37(2):88-102.

Younis JS, Naoum I, Salem N, Perlitz Y, Izhaki I. The impact of unilateral oophorectomy on ovarian reserve in assisted reproduction: a systematic review and meta-analysis. BJOG. 2018 Jan;125(1):26-35. doi: 10.1111/1471-0528.14913.






Research Article

How to Cite

Tanrıdan Okçu N, Külahcı Özgür, Dağlıoğlu G, Dağlıoğlu K, Nazik H. Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency. J Surg Med [Internet]. 2021 May 1 [cited 2024 Feb. 21];5(5):534-9. Available from: