Frequencies of glutathione S-transferase A1 rs3957357 polymorphism in a Turkish population

Zuhal Uçkun Şahinoğulları

doi:10.28982/josam.871071

Authors

Zuhal Uçkun Şahinoğulları https://orcid.org/0000-0002-3244-4103

DOI:

https://doi.org/10.28982/josam.871071

Keywords:

Glutathione S-transferases, GSTA1, rs3957357, Polymorphism, Turkish population

Abstract

Background/Aim: Glutathione-S-transferases (GSTs), a major group of phase II enzymes, play a significant role in the detoxification and metabolism of endogenous and exogenous compounds. The objective of the survey was to identify the distribution of genotype and allele frequencies of GSTA1 -69C>T (rs3957357) polymorphism in a healthy Turkish population and compare the determined frequencies with those in various populations. Methods: Polymerase chain reaction and restriction fragment length polymorphism methods were used to analyze GSTA1 -69C>T polymorphism in DNA samples of 105 healthy Turkish individuals. Results: The distribution of GSTA1 CC, CT, and TT genotype frequencies were 32.4%, 48.6% and 19.0%, respectively while the allele frequencies were 56.7% for C allele and 43.3% for T allele. The findings obtained were compared with the results of various populations. The frequencies of GSTA1 -69C>T polymorphism were similar to those of the African American population and the populations with White ancestry, but significantly different from those reported for the populations with Asian ancestry. Conclusion: To the best of our knowledge, this is the first study to present the frequencies of the GSTA1 -69C>T polymorphism among Turkish individuals. The findings of the current study may provide a perspective for further studies exploring the role of GSTA1 -69C>T polymorphism on predisposition to diverse illnesses such as cancer and may be used as a control group for such studies. In addition, this study might contribute to epidemiological and toxicogenetic investigations.

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Uckun Sahinogullari Z. Cytochrome P450 2A13 3375C