Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation

Ferda Keskin Çimen; Orhan Çimen; Durdu Altuner; Arif Burak Çekiç; Nezahat Kurt; Halis Süleyman

doi:10.28982/josam.858237

Authors

Ferda Keskin Çimen https://orcid.org/0000-0002-1844-0827
Orhan Çimen https://orcid.org/0000-0003-4723-5838
Durdu Altuner https://orcid.org/0000-0002-5756-3459
Arif Burak Çekiç https://orcid.org/0000-0002-1460-6949
Nezahat Kurt https://orcid.org/0000-0002-1685-5332
Halis Süleyman https://orcid.org/0000-0002-9239-4099

DOI:

https://doi.org/10.28982/josam.858237

Keywords:

Rutin, small intestine, ischemia, reperfusion, rat

Abstract

Background/Aim: The large amount of oxygen presented to the ischemic tissue in reperfusion causes the formation of excess free oxygen radicals and results in oxidative damage. Rutin is a flavonoid with potent antioxidant and anti-inflammatory effects. The aim of this study is to examine the effect of rutin on I/R-induced small intestinal (ileum) oxidative damage in rats. Methods: The animals were divided into three groups as follows: Intestinal ischemia-reperfusion (IIR), 50 mg/kg rutin+intestinal ischemia reperfusion (RIIR) and sham operation (Sham). Rutin was administered at a dose of 50 mg/kg by oral catheterization one hour prior to thiopental sodium anesthesia. Distilled water was administered with the same method to IIR and Sham groups as a solvent. To induce intestinal ischemia in RIIR and IIR groups, the superior mesenteric artery was suspended from the point where it left the aorta, and ischemia was induced for 45 minutes with the help of an atraumatic microvascular clamp followed by 60 minutes of reperfusion. Biochemical and histopathological examinations were performed on the dissected ileal tissues. Results: The amount of MDA and MPO activity increased, while tGSH levels and CAT activity decreased significantly in the intestinal tissue of the IIR group compared to sham group (P<0.001). Rutin treatment decreased the increase in MDA and MPO activity and increased the decrease of tGSH levels and CAT activity significantly compared to the IIR group (P<0.001). Histopathological changes such as PNL infiltration, edema, hemorrhage, and destruction were observed in the ileal tissue of the rats in the IIR group. However, there were no pathological findings in the RIIR group treated with rutin except for mildly dilated congested blood vessels. Conclusion: Rutin may be useful against intestinal I/R oxidative damage in clinical practice.

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