Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation

Authors

Keywords:

Rutin, small intestine, ischemia, reperfusion, rat

Abstract

Background/Aim: The large amount of oxygen presented to the ischemic tissue in reperfusion causes the formation of excess free oxygen radicals and results in oxidative damage. Rutin is a flavonoid with potent antioxidant and anti-inflammatory effects. The aim of this study is to examine the effect of rutin on I/R-induced small intestinal (ileum) oxidative damage in rats. Methods: The animals were divided into three groups as follows: Intestinal ischemia-reperfusion (IIR), 50 mg/kg rutin+intestinal ischemia reperfusion (RIIR) and sham operation (Sham). Rutin was administered at a dose of 50 mg/kg by oral catheterization one hour prior to thiopental sodium anesthesia. Distilled water was administered with the same method to IIR and Sham groups as a solvent. To induce intestinal ischemia in RIIR and IIR groups, the superior mesenteric artery was suspended from the point where it left the aorta, and ischemia was induced for 45 minutes with the help of an atraumatic microvascular clamp followed by 60 minutes of reperfusion. Biochemical and histopathological examinations were performed on the dissected ileal tissues. Results: The amount of MDA and MPO activity increased, while tGSH levels and CAT activity decreased significantly in the intestinal tissue of the IIR group compared to sham group (P<0.001). Rutin treatment decreased the increase in MDA and MPO activity and increased the decrease of tGSH levels and CAT activity significantly compared to the IIR group (P<0.001). Histopathological changes such as PNL infiltration, edema, hemorrhage, and destruction were observed in the ileal tissue of the rats in the IIR group. However, there were no pathological findings in the RIIR group treated with rutin except for mildly dilated congested blood vessels. Conclusion: Rutin may be useful against intestinal I/R oxidative damage in clinical practice.

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References

Dixon MF. The small intestine. In: Whitehead R (Eds). Gastrointestinal and Oesophageal Pathology. Livingstone: Curchill. 1995, 665-77.

Vejchapipat P, Williams SR, Spitz L, Pierro A. Intestinal metabolism after ischemia reperfusion. J Pediatr Surg. 2000;35:759-64. doi:10.1053/jpsu.2000.6062.

Suleyman H, Gul V, Hirik E. Ischemia-Reperfusion Damage. Erzincan Tıp Dergisi. 2018;1:54.

Belda-Antolí M, Padrón-Sanz C, Cejalvo-Lapeña D, Prieto-Moure B, Lloris-Cejalvo JM, Lloris-Carsí JM. Antioxidant potential of Himanthalia elongata for protection against ischemia reperfusion injury in the small bowel. Surgery. 2017;162:577-85. doi:10.1016/j.surg.2017.04.017.

Toyokuni S. Reactive oxygen species induced molecular damage and its application in pathology. Pathology international. 1999;49:91-102. doi:10.1046/j.1440-1827.1999.00829.x.

Saleh H, El-Shorbagy HM. Mechanism underlying methyl eugenol attenuation of intestinal ischemia/reperfusion injury. Appl Physiol Nutr Metab. 2017;42:1097-105. doi: 10.1139/apnm-2017-0043.

Harborne JB. Nature, distribution and function of plant flavonoids. Prog Clin Biol Res. 1986;213:15-24.

Ganeshpurkar A, Saluja AK. The pharmacological potential of rutin. Saudi Pharm J. 2017;25:149-64. doi: 10.1016/j.jsps.2016.04.025

Topal I, Akbulut UE, Cimen O, Kolkıran A, Akturan S, Cimen FK, et al. Effect of Rutin on Cisplatin-induced Small Intestine (jejunum) Damage in Rats. Int J Pharmacol. 2018;14:1136-44.

Demiryilmaz I, Turan MI, Kisaoglu A, Gulapoglu M, Yilmaz I, Suleyman H. Protective effect of nimesulide against hepatic ischemia/reperfusion injury in rats: effects on oxidant/antioxidants, DNA mutation and COX-1/COX-2 levels. Pharmacol Rep. 2014;66:647-52. doi: 10.1016/j.pharep.2014.02.015.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351-8.

Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol. 1982;78:206-9.

Sedlak J, Lindsay RH. Estimation of total, protein bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968;25:192-205.

Aebi H. Catalase in vitro Methods Enzymol 1984;105:121–6.

Granger D. Ischemia reperfusion injury: role of oxygen derived free radicals. Acta Physiol Scand Suppl. 1986;548:47-63.

Carden DL, Granger DN. Pathophysiology of ischaemia reperfusion injury. J Pathol. 2000;190:255-66. doi: 10.1002/(SICI)1096-9896(200002)190:3<255::AID-PATH526>3.0.CO;2-6.

Sasaki M, Joh T. Inflammation and Ischemia Reperfusion Injury in Gastrointestinal Tract and Antioxidant, Protective Agents. Journal of clinical biochemistry and nutrition. 2007;40:1-12. doi: 10.3164/jcbn.40.1.

Paradowski PT, Zeman K. [Pentoxifylline]. Postepy Hig Med Dosw. 1995;49:201-20.

Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper's biochemistry: a LANGE medicinal book. McGraw-Hill; 2000.

Kisaoglu A, Borekci B, Yapca OE, Bilen H, Suleyman H. Tissue damage and oxidant/antioxidant balance. Eurasian J Med. 2013;45:47. doi: 10.5152/eajm.2013.08.

Nayki C, Nayki U, Keskin Cimen F, Kulhan M, Yapca OE, Kurt N, et al. The effect of rutin on ovarian ischemia reperfusion injury in a rat model. Gynecol Endocrinol. 2018;34:809-14. doi: 10.1080/09513590.2018.1450378.

Liu Y, Gou L, Fu X, Li S, Lan N, Yin X. Protective effect of rutin against acute gastric mucosal lesions induced by ischemia reperfusion. Pharm Biol. 2013;51:914-9. doi: 10.3109/13880209.2013.771375.

Kirkman HN, Gaetani GF. Catalase: a tetrameric enzyme with four tightly bound molecules of NADPH. P Natl Acad Sci USA. 1984;81:4343-7. doi: 10.1073/pnas.81.14.4343

Scibior D, Czeczot H. Katalaza–budowa, właściwości, funkcje [Catalase: structure, properties, functions]. Postepy Hig Med Dosw (Online). 2006;60:170-80.

Arslan A, Ozcicek A, Suleyman B, Coban TA, Cimen FK, Nalkiran HS, et al. Effects of nimesulide on the small intestine mucositis induced by methotrexate in rats. Exp Anim. 2016:15-0122. doi: 10.1538/expanim.15-0122.

Tuboly E, Futakuchi M, Varga G, Érces D, Tőkés T, Mészáros A, et al. C5a inhibitor protects against ischemia/reperfusion injury in rat small intestine. Microbiol Immunol. 2016;60:35-46. doi: 10.1111/1348-0421.12338.

Zheng X, Mao Y, Cai J, Li Y, Liu W, Sun P, et al. Hydrogen rich saline protects against intestinal ischemia/reperfusion injury in rats. Free Radic Res. 2009;43:478-84. doi: 10.1080/10715760902870603.

Teke Z, Bostanci EB, Yenisey C, Sacar M, Simsek NG, Akoglu M. Caffeic acid phenethyl ester alleviates mesenteric ischemia/reperfusion injury. J Invest Surg. 2012;25:354-65. doi: 10.3109/08941939.2012.677968.

Soydan G, Sokmensuer C, Kilinc K, Tuncer M. The effects of sildenafil on the functional and structural changes of ileum induced by intestinal ischemia reperfusion in rats. Eur J Pharmacol. 2009;610:87-92. doi: 10.1016/j.ejphar.2009.03.038.

Gordeeva AE, Temnov AA, Charnagalov AA, Sharapov MG, Fesenko EE, Novoselov VI. Protective Effect of Peroxiredoxin 6 in Ischemia/Reperfusion Induced Damage of Small Intestine. Dig Dis Sci. 2015;60:3610-9.

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Published

2021-01-01

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Research Article

How to Cite

1.
Keskin Çimen F, Çimen O, Altuner D, Çekiç AB, Kurt N, Süleyman H. Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation. J Surg Med [Internet]. 2021 Jan. 1 [cited 2022 Oct. 7];5(1):26-30. Available from: https://jsurgmed.com/article/view/858237