High-risk human papillomavirus infection prevalence in non-malignant tonsillar tissue: A single-center cross-sectional study: Human papillomavirus infection prevalence

Gül  Bayram; Tugce Simsek; Selin Hazir; Fatma Zeynep   Ozen; Mehmet Mustafa  Erdogan; Sinan Seyhan; Hasan  Kazaz; Erkan  Tezcan

doi:10.28982/josam.7983

Authors

Gül Bayram Mersin University, Vocational School of Health Services, Mersin, Turkey https://orcid.org/0000-0002-4154-8596
Tugce Simsek Department of Otorhinolaryngology and Head and Neck Surgery, Private Clinic, İstanbul, Turkey https://orcid.org/0000-0002-1425-8833
Selin Hazir Mersin University, Vocational School of Health Services, Mersin, Turkey https://orcid.org/0000-0002-0714-5929
Fatma Zeynep Ozen Department of Medical Pathology, Amasya University Sabuncuoglu Serefeddin Training and Research Hospital, Amasya, Turkey https://orcid.org/0000-0003-2468-8375
Mehmet Mustafa Erdogan Department of Otorhinolaryngology and Head and Neck Surgery, Amasya University Sabuncuoglu Serefeddin Training and Research Hospital, Amasya, Turkey https://orcid.org/0000-0001-9955-0704
Sinan Seyhan Department of Otorhinolaryngology and Head and Neck Surgery, Amasya University Sabuncuoglu Serefeddin Training and Research Hospital, Amasya, Turkey https://orcid.org/0000-0002-8993-8008
Hasan Kazaz Department of Otorhinolaryngology and Head and Neck Surgery, Trabzon Kanuni Training and Research Hospital, Trabzon, Turkey https://orcid.org/0000-0002-7889-2315
Erkan Tezcan Department of Otorhinolaryngology and Head and Neck Surgery, Çanakkale Onsekiz Mart University Medical Faculty Hospital, Çanakkale, Turkey https://orcid.org/0000-0003-1413-1266

DOI:

https://doi.org/10.28982/josam.7983

Keywords:

Papillomavirus, HPV type 16, non-malignant tonsil, Real-time PCR, high-risk human papillomavirus.

Abstract

Background/Aim: The prevalence of human papillomavirus (HPV) in non-malignant tonsils can vary according to geographical location, age group, and risk factors. Some studies have found a relatively low prevalence of HPV, while other studies have found higher rates in non-malignant tonsils. The presence of HPV in non-malignant tonsils may be associated with precursor lesions that have the potential to develop into cancer. The aim of the current study was to detect the prevalence of HPV and p16 (one of the HPV types) in non-malignant tonsils and determine the existence of HPV in tonsil tissue using molecular and histological techniques.

Methods: One hundred-three samples from non-malignant tonsils and one sample from squamous cell carcinoma of the tonsils were analyzed for the prevalence of HPV using molecular and histological methods. Real-time polymerase chain reaction (qPCR) was performed to detect HPV in the tissue samples.

Results: HPV was not found in any tissue specimens based on histopathological and p16 immunohistochemical evaluations. HPV was not detected in all tissue samples using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR).

Conclusions: In our study of one hundred and four patients, HPV and p16 were not genetically detected in the tonsils that underwent surgery for reasons other than cancer. Hence, more comprehensive studies can contribute to evaluating the relationship between benign tonsil tissue and HPV infection, potentially leading to improved diagnostic and preventative measures.

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References

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015;136(5):E359-E386. doi: 10.1002/ijc.29210. DOI: https://doi.org/10.1002/ijc.29210

Mehanna H, Beech T, Nicholson T, El-Hariry I, McConkey C, Paleri V, et al. Prevalence of human papillomavirus in oropharyngeal and nonoropharyngeal head and neck cancer--systematic review and meta-analysis of trends by time and region. Head Neck. 2013;35(5):747-55. doi: 10.1002/hed.22015. DOI: https://doi.org/10.1002/hed.22015

Mooren JJ, Gültekin SE, Straetmans JM, Haesevoets A, Peutz-Kootstra CJ, Huebbers CU, et al. P16(INK4A) immunostaining is a strong indicator for high-risk-HPV-associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low-risk-HPV-infection in head and neck papillomas and laryngeal dysplasias. Int J Cancer. 2014;134(9):2108-17. doi: 10.1002/ijc.28534. DOI: https://doi.org/10.1002/ijc.28534

Gillison ML, Broutian T, Pickard RK, Tong ZY, Xiao W, Kahle L, et al. Prevalence of oral HPV infection in the United States, 2009-2010. JAMA. 2012;307(7):693-703. doi: 10.1001/jama.2012.101. DOI: https://doi.org/10.1001/jama.2012.101

Tam S, Fu S, Xu L, Krause KJ, Lairson DR, Miao H, et al. The epidemiology of oral human papillomavirus infection in healthy populations: A systematic review and meta-analysis. Oral Oncol. 2018;82:91-9. doi: 10.1016/j.oraloncology.2018.04.005. DOI: https://doi.org/10.1016/j.oraloncology.2018.04.005

Ilmarinen T, Munne P, Hagström J, Haglund C, Auvinen E, Virtanen EI, et al. Prevalence of high-risk human papillomavirus infection and cancer gene mutations in nonmalignant tonsils. Oral Oncol. 2017;73:77-82. doi: 10.1016/j.oraloncology.2017.08.010. DOI: https://doi.org/10.1016/j.oraloncology.2017.08.010

Chaturvedi AK, Engels EA, Pfeiffer RM, Hernandez BY, Xiao W, Kim E, et al. Human papillomavirus and rising oropharyngeal cancer incidence in the United States. J Clin Oncol. 2011;29(32):4294-301. doi: 10.1200/JCO.2011.36.4596. DOI: https://doi.org/10.1200/JCO.2011.36.4596

de Araujo MR, De Marco L, Santos CF, Rubira-Bullen IR, Ronco G, Pennini I, et al. GP5+/6+ SYBR Green methodology for simultaneous screening and quantification of human papillomavirus. J Clin Virol. 2009 Jun;45(2):90-5. doi: 10.1016/j.jcv.2009.03.020. DOI: https://doi.org/10.1016/j.jcv.2009.03.020

Qu W, Jiang G, Cruz Y, Chang CJ, Ho GY, Klein RS, Burk RD. PCR detection of human papillomavirus: comparison between MY09/MY11 and GP5+/GP6+ primer systems. J Clin Microbiol. 1997;35(6):1304-10. doi: 10.1128/jcm.35.6.1304-1310.1997. DOI: https://doi.org/10.1128/jcm.35.6.1304-1310.1997

Syrjänen S. Current concepts on human papillomavirus infections in children. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2010;118(6-7):494–509. doi: 10.1111/j.1600-0463.2010.02620.x. DOI: https://doi.org/10.1111/j.1600-0463.2010.02620.x

Heck JE, Berthiller J, Vaccarella S, Winn DM, Smith EM, Shan'gina O, et al. Sexual behaviours and the risk of head and neck cancers: a pooled analysis in the International Head and Neck Cancer Epidemiology (INHANCE) consortium. Int J Epidemiol. 2010;39(1):166-81. doi: 10.1093/ije/dyp350. DOI: https://doi.org/10.1093/ije/dyp350

Chen R, Sehr P, Waterboer T, Leivo I, Pawlita M, Vaheri A, Aaltonen LM. Presence of DNA of human papillomavirus 16 but no other types in tumor-free tonsillar tissue. J Clin Microbiol. 2005;43(3):1408-10. doi: 10.1128/JCM.43.3.1408-1410.2005. DOI: https://doi.org/10.1128/JCM.43.3.1408-1410.2005

Palmer E, Newcombe RG, Green AC, Kelly C, Noel Gill O, Hall G, Fiander AN. Human papillomavirus infection is rare in nonmalignant tonsil tissue in the UK: implications for tonsil cancer precursor lesions. Int J Cancer. 2014;135(10):2437-43. doi: 10.1002/ijc.28886. DOI: https://doi.org/10.1002/ijc.28886

Xue XC, Chen XP, Yao WH, Zhang Y, Sun GB, Tan XJ. Prevalence of human papillomavirus and Epstein-Barr virus DNA in Chinese children with tonsillar and/or adenoidal hypertrophy. J Med Virol. 2014;86(6):963-7. doi: 10.1002/jmv.23894. DOI: https://doi.org/10.1002/jmv.23894

Rusan M, Klug TE, Henriksen JJ, Bonde JH, Fuursted K, Ovesen T. Prevalence of tonsillar human papillomavirus infections in Denmark. Eur Arch Otorhinolaryngol. 2015;272(9):2505-12. doi: 10.1007/s00405-014-3225-x. DOI: https://doi.org/10.1007/s00405-014-3225-x

Klingenberg B, Hafkamp HC, Haesevoets A, Manni JJ, Slootweg PJ, Weissenborn SJ, et al. p16 INK4A overexpression is frequently detected in tumour-free tonsil tissue without association with HPV. Histopathology. 2010;56(7):957-67. DOI: https://doi.org/10.1111/j.1365-2559.2010.03576.x

Sisk J, Schweinfurth, JM, Wang, XT, Chong K. Presence of human papillomavirus DNA in tonsillectomy specimens. The Laryngoscope. 2006;116(8):1372–4. doi: 10.1111/j.1365-2559.2010.03576.x. DOI: https://doi.org/10.1097/01.mlg.0000225973.21736.bc

Wojtera M, Paradis J, Husein M, Nichols AC, Barrett JW, Salvadori MI, Strychowsky JE. The prevalence of human papillomavirus in pediatric tonsils: a systematic review of the literature. J Otolaryngol Head Neck Surg. 2018;47(1):8. doi: 10.1186/s40463-018-0255-1. DOI: https://doi.org/10.1186/s40463-018-0255-1

Ribeiro KM, Alvez JM, Pignatari SS, Weckx LL. Detection of human papilloma virus in the tonsils of children undergoing tonsillectomy. Braz J Infect Dis. 2006;10(3):165-8. doi: 10.1590/s1413-86702006000300002. DOI: https://doi.org/10.1590/S1413-86702006000300002

Duray A, Descamps G, Bettonville M, Sirtaine N, Ernoux-Neufcoeur P, Guenin S, Mouallif M, et al. High prevalence of high-risk human papillomavirus in palatine tonsils from healthy children and adults. Otolaryngol Head Neck Surg. 2011;145(2):230-5. doi: 10.1590/s1413-86702006000300002. DOI: https://doi.org/10.1177/0194599811402944

Bruni L, Diaz M, Castellsagué X, Ferrer E, Bosch FX, de Sanjosé S. Cervical human papillomavirus prevalence in 5 continents: meta-analysis of 1 million women with normal cytological findings. J Infect Dis. 2010;202(12):1789-99. doi: 10.1086/657321. DOI: https://doi.org/10.1086/657321

Gillison ML, Castellsagué X, Chaturvedi A, Goodman MT, Snijders P, Tommasino M, et al. Eurogin Roadmap: comparative epidemiology of HPV infection and associated cancers of the head and neck and cervix. Int J Cancer. 2014;134(3):497-507. doi: 10.1002/ijc.28201. DOI: https://doi.org/10.1002/ijc.28201

Fakhry C, Rosenthal BT, Clark DP, Gillison ML. Associations between oral HPV16 infection and cytopathology: evaluation of an oropharyngeal "pap-test equivalent" in high-risk populations. Cancer Prev Res (Phila). 2011;4(9):1378-84. doi: 10.1158/1940-6207. DOI: https://doi.org/10.1158/1940-6207.CAPR-11-0284

Olthof NC, Straetmans JM, Snoeck R, Ramaekers FC, Kremer B, Speel EJ. Next-generation treatment strategies for human papillomavirus-related head and neck squamous cell carcinoma: where do we go? Rev Med Virol. 2012;22(2):88-105. doi: 10.1002/rmv.714. DOI: https://doi.org/10.1002/rmv.714

Alos L, Moyano S, Nadal A, Alobid I, Blanch JL, Ayala E, et al. Human papillomaviruses are identified in a subgroup of sinonasal squamous cell carcinomas with favorable outcome. Cancer. 2009;115(12):2701-9. doi: 10.1002/cncr.24309. DOI: https://doi.org/10.1002/cncr.24309

Larque AB, Hakim S, Ordi J, Nadal A, Diaz A, del Pino M, Marimon L et al. High-risk human papillomavirus is transcriptionally active in a subset of sinonasal squamous cell carcinomas. Mod Pathol. 2014;27(3):343-51. doi: 10.1038/modpathol.2013.155. DOI: https://doi.org/10.1038/modpathol.2013.155

Bishop JA, Guo TW, Smith DF, Wang H, Ogawa T, Pai SI, et al. Human papillomavirus-related carcinomas of the sinonasal tract. Am J Surg Pathol. 2013;37(2):185-92. doi: 10.1097/PAS.0b013e3182698673. DOI: https://doi.org/10.1097/PAS.0b013e3182698673

Thavaraj S. Human papillomavirus-associated neoplasms of the sinonasal tract and nasopharynx. Semin Diagn Pathol. 2016;33(2):104-11. doi: 10.1053/j.semdp.2015.09.011. DOI: https://doi.org/10.1053/j.semdp.2015.09.011