Self-reported occupational exposure and its association with sperm DNA fragmentation in infertile men
Occupation and sperm DNA fragmentation
Keywords:
male infertility, occupational exposure, sperm DNA fragmentationAbstract
Background/Aim: Sperm quality has experienced a decline in recent years, with this issue being particularly pronounced in industrialized nations, suggesting a potential link to occupational exposures. Evaluating sperm DNA fragmentation can yield valuable insights into male fertility, although its association with occupational exposures remains less well-established. Our study aimed to investigate the relationship between self-reported occupational exposures and sperm DNA fragmentation in infertile men.
Methods: This retrospective cohort study involved 391 infertile men who sought fertility treatment at a university clinic between 2017 and 2020. A brief questionnaire was administered to collect data on patients’ demographic characteristics, medical history, occupation, and exposure types. In this comparative study, patients were categorized into two groups based on their occupational exposures (the unexposed and exposed groups). The exposed group was further sub-grouped according to their specific exposure types, which included cement, solvents, metals, pesticides, mechanical vibration, and heat. The primary outcome in this study was assessed using the terminal deoxynucleotidyl transferase-mediated nick end-labeling test (TUNEL), with results expressed as the sperm DNA fragmentation index (DFI).
Results: Patients in the exposed group exhibited a significantly higher sperm DFI compared to those in the unexposed group (14 [17] vs. 8 [9], P<0.001). After accounting for potential confounding factors, our results demonstrated that several occupational exposure factors significantly increased the risk of elevated sperm DFI (>15%) levels, including solvents (odds ratio (OR) 8.2, 95% confidence interval (CI) 3.6–18.5, P<0.001), metals (OR: 2.2, 95% CI: 1.0–4.7, P=0.048), pesticides (OR: 14.6, 95% CI: 1.6–130.7, P=0.016), mechanical vibration (OR: 2.6, 95% CI: 1.5–4.6, P<0.001), and heat (OR: 6.4, 95% CI: 1.7–23.5, P=0.005).
Conclusion: The findings of our study corroborate earlier research suggesting that occupational exposures may have adverse effects on sperm DNA fragmentation in men. The identification and management of such exposures as part of routine clinical practice could offer a complementary approach to enhancing infertility treatment outcomes.
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Esteves SC, Sánchez-Martín F, Sánchez-Martín P, Schneider DT, Gosálvez J. Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm. Fertil Steril. 2015;104:1398–405. doi: 10.1016/j.fertnstert.2015.08.028. DOI: https://doi.org/10.1016/j.fertnstert.2015.08.028
Panner Selvam MK, Ambar RF, Agarwal A, Henkel R. Etiologies of sperm DNA damage and its impact on male infertility. Andrologia. 2020;00:e13706. doi: 10.1111/and.13706. DOI: https://doi.org/10.1111/and.13706
Bonde JPE. Occupational causes of male infertility. Curr Opin Endocrinol Diabetes Obes. 2013;20:234–9. doi: 10.1097/MED.0b013e32835f3d4b. DOI: https://doi.org/10.1097/MED.0b013e32835f3d4b
Kumar S. Occupational exposure associated with reproductive dysfunction. J Occup Health. 2004;46:1-19. doi: 10.1539/joh.46.1. DOI: https://doi.org/10.1539/joh.46.1
Figa-Talamanca I, Traina ME, Urbani E. Occupational exposures to metals, solvents and pesticides: Recent evidence on male reproductive effects and biological markers. Occup Med. 2001;51:174–88. doi: 10.1093/occmed/51.3.174. DOI: https://doi.org/10.1093/occmed/51.3.174
Martenies SE, Perry MJ. Environmental and occupational pesticide exposure and human sperm parameters: a systematic review. Toxicology. 2013;307:66-73. doi: 10.1016/j.tox.2013.02.005. DOI: https://doi.org/10.1016/j.tox.2013.02.005
Sharma R, Biedenharn KR, Fedor JM, Agarwal A. Lifestyle factors and reproductive health: taking control of your fertility. Reprod Biol Endocrinol. 2013;11:66. doi: 10.1186/1477-7827-11-66. DOI: https://doi.org/10.1186/1477-7827-11-66
Evenson DP, Wixon R. Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA). Toxicol Appl Pharmacol. 2005;207(2):532-7. doi: 10.1016/j.taap.2005.03.021. DOI: https://doi.org/10.1016/j.taap.2005.03.021
Tielemans E, Heederik D, Burdorf A, Vermeulen R, Veulemans H, Kromhout H, et al. Assessment of occupational exposures in a general population: comparison of different methods. Occup Environ Med. 1999;56:145-51. doi: 10.1136/oem.56.3.145. DOI: https://doi.org/10.1136/oem.56.3.145
Caliskan Z, Kucukgergin C, Aktan G, Kadioglu A, Ozdemirler G. Evaluation of sperm DNA fragmentation in male infertility. Andrologia. 2022;54(11):e14587. doi: 10.1111/and.14587. DOI: https://doi.org/10.1111/and.14587
De Fleurian G, Perrin J, Ecochard R, Dantony E, Lanteaume A, Achard V, et al. Occupational exposures obtained by questionnaire in clinical practice and their association with semen quality. J Androl. 2009;30:566. doi: 10.2164/jandrol.108.005918. DOI: https://doi.org/10.2164/jandrol.108.005918
Ianos O, Sari-Minodier I, Villes V, Lehucher-Michel MP, Loundou A, Perrin J. Meta-Analysis Reveals the Association Between Male Occupational Exposure to Solvents and Impairment of Semen Parameters. J Occup Environ Med. 2018;60(10):e533-e542. doi: 10.1097/JOM.0000000000001422. DOI: https://doi.org/10.1097/JOM.0000000000001422
Marchetti F, Eskenazi B, Weldon RH, Li G, Zhang L, Rappaport SM, et al. Occupational exposure to benzene and chromosomal structural aberrations in the sperm of Chinese men. Environ Health Perspect. 2012;120(2):229-34. doi: 10.1289/ehp.1103921. DOI: https://doi.org/10.1289/ehp.1103921
Irnandi DF, Hinting A, Yudiwati R. DNA fragmentation of sperm in automobile painters. Toxicol Ind Health. 2021;37(4):182-8. doi: 10.1177/0748233721989892. DOI: https://doi.org/10.1177/0748233721989892
Migliore L, Naccarati A, Zanello A, Scarpato R, Bramanti L, Mariani M. Assessment of sperm DNA integrity in workers exposed to styrene. Hum Reprod. 2002;17(11): 2912–8. doi: 10.1093/humrep/17.11.2912. DOI: https://doi.org/10.1093/humrep/17.11.2912
Jeng HA, Pan CH, Chao MR, Chiu CC, Zhou G, Chou CK, et al. Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons. Int J Occup Med Environ Health. 2016;29(6):915-26. doi: 10.13075/ijomeh.1896.00598. DOI: https://doi.org/10.13075/ijomeh.1896.00598
Jurewicz J, Radwan M, Sobala W, Radwan P, Bochenek M, Hanke W. Effects of occupational exposure - is there a link between exposure based on an occupational questionnaire and semen quality? Syst Biol Reprod Med. 2014;60(4): 227–33. doi: 10.3109/19396368.2014.907837. DOI: https://doi.org/10.3109/19396368.2014.907837
Jamalan M, Ghaffari MA, Hoseinzadeh P, Hashemitabar M, Zeinali M. Human sperm quality and metal toxicants: protective effects of some flavonoids on male reproductive function. Int J Fertil Steril. 2016;10(2):215–23. doi: 10.22074/ijfs.2016.4912.
Zhou Y, Fu XM, He DL, Zou XM, Wu CQ, Guo WZ, et al. Evaluation of urinary metal concentrations and sperm DNA damage in infertile men from an infertility clinic. Environ Toxicol Pharmacol. 2016;45:68-73. doi: 10.1016/j.etap.2016.05.020. DOI: https://doi.org/10.1016/j.etap.2016.05.020
Quintanilla-Vega B, Hoover D, Bal W, Silbergeld EK, Waalkes MP, Anderson LD. Lead effects on protamine-DNA binding. Am J Ind Med. 2000;38:324–9. doi: 10.1002/1097-0274(200009)38:3. DOI: https://doi.org/10.1002/1097-0274(200009)38:3<324::AID-AJIM12>3.0.CO;2-R
Jeng HA, Sikdar S, Huang YL, Pan CH. Mixture analysis of associations between exposure to low levels of multiple metals and semen quality and sperm DNA integrity. J Environ Sci Health - Part A, Toxic/Hazard. 2022;57(4):318–26. doi: 10.1080/10934529.2022.2061256. DOI: https://doi.org/10.1080/10934529.2022.2061256
Perry MJ. Effects of environmental and occupational pesticide exposure on human sperm: a systematic review. Hum Reprod Update. 2008;14:233-42. doi: 10.1093/humupd/dmm039. DOI: https://doi.org/10.1093/humupd/dmm039
Mehrpour O, Karrari P, Zamani N, Tsatsakis AM, Abdollahi M. Occupational exposure to pesticides and consequences on male semen and fertility: a review. Toxicol Lett. 2014;230:146-56. doi: 10.1016/j.toxlet.2014.01.029. DOI: https://doi.org/10.1016/j.toxlet.2014.01.029
Bian Q, Xu LC, Wang SL, Xia YK, Tan LF, Chen JF, et al. Study on the relation between occupational fenvalerate exposure and spermatozoa DNA damage of pesticide factory workers. Occup Environ Med. 2004;61:999–1005. doi: 10.1136/oem.2004.014597. DOI: https://doi.org/10.1136/oem.2004.014597
Xia Y, Cheng S, Bian Q, Xu L, Collins MD, Chang HC, et al. Genotoxic effects on spermatozoa of carbaryl-exposed workers. Toxicol Sci. 2005;85:615–23. doi: 10.1093/toxsci/kfi066. DOI: https://doi.org/10.1093/toxsci/kfi066
Gill K, Jakubik J, Kups M, Rosiak-Gill A, Kurzawa R, Kurpisz M, et al. The impact of sedentary work on sperm nuclear DNA integrity. Folia Histochem Cytobiol. 2019;57:15–22. doi: 10.5603/FHC.a2019.0002. DOI: https://doi.org/10.5603/FHC.a2019.0002
Durairajanayagam D, Sharma RK, du Plessis SS, Agarwal A. Testicular Heat Stress and Sperm Quality. In: du Plessis S, Agarwal A, Sabanegh Jr E, eds. Male Infertility. New York: Springer; 2017. Pp. 105-25. doi: 10.1007/978-1-4939-1040-3_8. DOI: https://doi.org/10.1007/978-1-4939-1040-3_8
Bujan L, Daudin M, Charlet JP, Thonneau P, Mieusset R. Increase in scrotal temperature in car drivers. Hum Reprod. 2000;15(6):1355–7. doi: 10.1093/humrep/15.6.1355. DOI: https://doi.org/10.1093/humrep/15.6.1355
Eisenberg ML, Chen Z, Ye A, Buck Louis GM. Relationship between physical occupational exposures and health on semen quality: data from the Longitudinal Investigation of Fertility and the Environment (LIFE) Study. Fertil Steril. 2015;103:1271–7. doi: 10.1016/j.fertnstert.2015.02.010. DOI: https://doi.org/10.1016/j.fertnstert.2015.02.010
Daoud S, Sellami A, Bouassida M, Kebaili S, Ammar Keskes L, Rebai T, et al. Routine assessment of occupational exposure and its relation to semen quality in infertile men: a cross-sectional study. Turk J Med Sci. 2017;47(3):902–7. doi: 10.3906/sag-1605-47. DOI: https://doi.org/10.3906/sag-1605-47
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Copyright (c) 2023 Zeynep Caliskan , Canan Kucukgergin, Gulsan Aktan, Nurgul Bulut, Gul Ozdemirler
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