The protective effect of hydroxytyrosol on the heart in rats fed corn syrup: The role of spexin, pentraxin-3

The protective effect of hydroxytyrosol on heart

Authors

Keywords:

corn syrup, hydroxytyrosol, spexin, pentraxin-3

Abstract

Background/Aim: Increased consumption of corn syrup has been linked to various metabolic diseases. The Mediterranean diet, one of the healthiest known diets, is renowned for its cardioprotective effects. We investigated the possible roles of new molecules such as spexin (SPX) and pentraxin-3 (PTX-3) in the protective effect of hydroxytyrosol (HT), one of the primary main components of olive oil, in rats fed corn syrup.

Methods: The animals were divided into four groups of n=6 rats each: Group I (Control), Group II (HT), Group III (Corn Syrup), and Group IV (Corn Syrup+HT). The rats were given 30% corn syrup with drinking water for 6 weeks. Liquid containing HT (4 ml/kg/day) was applied by oral gavage alone and together with corn syrup for 6 weeks. SPX and PTX-3 were examined histopathologically in the animals’ heart tissue after the rats were sacrificed, and histoscores were created for SPX and PTX-3 immunoreactivity. The data were analyzed using both one-way ANOVA and Tukey’s HSD test.

Results: We detected a decrease in SPX (P<0.001) and an increase in PTX-3 (P=0.013) in the Corn Syrup group compared with the Control group. After HT treatment, an increase in SPX (P=0.025) and a decrease in PTX-3 (P<0.001) were detected. There were no differences between the HT and Control groups.

Conclusion: The protective effects of HT against heart damage might be conferred via SPX and PTX-3. These molecules are considered to be important target molecules involved in the diagnosis and treatment of metabolic diseases.

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References

Madero M, Perez-Pozo SE, Jalal D, Johnson RJ, Sanchez-Lozada LG. Dietary fructose and hypertension. Curr Hypertens Rep. 2011;13(1):29-35. doi: 10.1007/s11906-010-0163-x. DOI: https://doi.org/10.1007/s11906-010-0163-x

Johnson RJ, Segal MS, Sautin Y, Nakagawa T, Feig DI, Kang DH, et al. Potential role of sugar (fructose) in the epidemic of hypertension, obesity and the metabolic syndrome, diabetes, kidney disease, and cardiovascular disease. Am J Clin Nutr. 2007;86(4):899-906. doi: 10.1093/ajcn/86.4.899.

Kizhner T, Werman MJ. Long-term fructose intake: biochemical consequences and altered renal histology in the male rat. Metabolism. 2002;51(12):1538-47. doi: 10.1053/meta.2002.36306. DOI: https://doi.org/10.1053/meta.2002.36306

Tappy L, Le KA, Tran C, Paquot N. Fructose and metabolic diseases: New findings, new questions. Nutrition. 2010;26(11-12):1044-9. doi: 10.1016/j.nut.2010.02.014. DOI: https://doi.org/10.1016/j.nut.2010.02.014

Sánchez-Lozada LG, Tapia E, Jiménez A, Bautista P, Cristóbal M, Nepomuceno T, et al. Fructose-induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats. Am J Physiol Renal Physiol. 2007;292(1):F423-9. doi: 10.1152/ajprenal.00124.2006. DOI: https://doi.org/10.1152/ajprenal.00124.2006

Mellor KM, Ritchie RH, Davidoff AJ, Delbridge LM. Elevated dietary sugar and the heart: experimental models and myocardial remodeling. Can J Physiol Pharmacol. 2010;88(5):525-40. doi: 10.1139/y10-005. DOI: https://doi.org/10.1139/Y10-005

Malik VS, Popkin BM, Bray GA, Despres JP, Willett WC, Hu FB. Sugar-sweetened beverages and risk of metabolic syndrome and type 2 diabetes: a meta-analysis. Diabetes Care. 2010;33(11):2477-83. doi: 10.2337/dc10-1079. DOI: https://doi.org/10.2337/dc10-1079

Boghossian NS, Yeung EH, Mumford SL, Zhang C, Gaskins AJ, Wactawski-Wende J, et al. Adherence to the Mediterranean diet and body fat distribution in reproductive aged women. Eur J Clin Nutr. 2013;67(3):289-94. doi: 10.1038/ejcn.2013.4. DOI: https://doi.org/10.1038/ejcn.2013.4

Bertoli S, Leone A, Vignati L, Bedogni G, Martinez-Gonzalez MA, Bes-Rastrollo M, et al. Adherence to the Mediterranean diet is inversely associated with visceral abdominal tissue in Caucasian subjects. Clin Nutr 2015;34(6):1266-72. doi: 10.1016/j.clnu.2015.10.003. DOI: https://doi.org/10.1016/j.clnu.2015.10.003

Bendall CL, Mayr HL, Opie RS, Bes-Rastrollo M, Itsiopoulos C, Thomas CJ. Central obesity and the Mediterranean diet: A systematic review of intervention trials. Crit Rev Food Sci Nutr. 2018;58(18):3070-84. doi: 10.1080/10408398.2017.1351917. DOI: https://doi.org/10.1080/10408398.2017.1351917

Tripoli E, Giammanco M, Tabacchi G, Di Majo D, Giammanco S, La Guardia M. The phenolic compounds of olive oil: structure, biological activity and beneficial effects on human health. Nutr Res Rev. 2005;18(1):98-112. doi: 10.1079/NRR200495. DOI: https://doi.org/10.1079/NRR200495

Mirabeau O, Perlas E, Severini C, Audero E, Gascuel O, Possenti R, et al. Identification of novel peptide hormones in the human proteome by hidden Markov model screening. Genome Res. 2007;17(3):320–7. doi: 10.1101/gr.5755407. DOI: https://doi.org/10.1101/gr.5755407

Wan B, Wang XR, Zhou YB, Zhang X, Huo K, Han ZG. C12ORF39, a novel secreted protein with a typical amidation processing signal. Biosci Rep. 2009;30(1):1-10. doi: 10.1042/BSR20080156. DOI: https://doi.org/10.1042/BSR20080156

Wong MK, Sze KH, Chen T, Cho CK, Law HC, Chu IK, et al. Goldfish spexin: solution structure and novel function as a satiety factor in feeding control. Am J Physiol Endocrinol Metab. 2013;305(3):E348-66. doi: 10.1152/ajpendo.00141.2013. DOI: https://doi.org/10.1152/ajpendo.00141.2013

Porzionato A, Rucinski M, Macchi V, Stecco C, Malendowicz LK, de Caro R. Spexin expression in normal rat tissues. J Histochem Cytochem. 2010;58(9):825–37. doi: 10.1369/jhc.2010.956300 DOI: https://doi.org/10.1369/jhc.2010.956300

Ma A, Bai J, He M, Wong AOL. Spexin as a neuroendocrine signal with emerging functions. Gen Comp Endocrinol. 2018;265:90–6. doi: 10.1016/j.ygcen.2018.01.015. DOI: https://doi.org/10.1016/j.ygcen.2018.01.015

Canas JA, Sweeten S, Balagopal PB. Biomarkers for cardiovascular risk in children. Curr Opin Cardiol. 2013;28(2):103–14. doi: 10.1097/HCO.0b013e32835dd0ce. DOI: https://doi.org/10.1097/HCO.0b013e32835dd0ce

Robinson K, Prins J, Venkatesh B. Clinical review: Adiponectin biology and its role in inflammation and critical illness. Crit Care. 2011;15(2):221. doi: 10.1186/cc10021. DOI: https://doi.org/10.1186/cc10021

Maroni P, Bendinelli P, Piccoletti R. Intracellular signal transduction pathways induced by leptin in C2C12 cells. Cell Biology International. 2005;29:542-50. doi: 10.1016/j. cellbi.2005.03.008 DOI: https://doi.org/10.1016/j.cellbi.2005.03.008

Matsuzawa Y, Funahashi T, Kihara S, Shimomura I. Adiponectin and metabolic syndrome. Arterioscler Thromb Vasc Biol. 2004;24(1):29-33. doi: 10.1161/01.ATV.0000099786.99623.EF. DOI: https://doi.org/10.1161/01.ATV.0000099786.99623.EF

Fu Y. Adiponectin signaling and metabolic syndrome. Prog Mol Biol Transl Sci. 2014;121:293-319. doi: 10.1016/B978-0-12-800101-1.00009-0. DOI: https://doi.org/10.1016/B978-0-12-800101-1.00009-0

Shimano M, Shibata R, Tsuji Y, Kamiya H, Uchikawa T, Harata S. Circulating adiponectin levels in patients with atrial fibrillation. Circ J. 2008;72(7):1120-4. doi: 10.1253/circj.72.1120. DOI: https://doi.org/10.1253/circj.72.1120

Kardas F, Akın L, Kurtoglu S, Kendirci M, Kardas Z. Plasma Pentraxin 3 as a biomarker of metabolic syndrome. Indian J Pediatr. 2015;82(1):35-8. doi: 10.1007/s12098-014-1542-0. DOI: https://doi.org/10.1007/s12098-014-1542-0

Gün A, Özer MK, Bilgiç S, Kocaman N, Ozan G. Effect of Caffeic Acid Phenethyl Ester on Vascular Damage Caused by Consumption of High Fructose Corn Syrup in Rat. Oxid Med Cell Longev. 2016;2016:3419479. doi: 10.1155/2016/3419479. DOI: https://doi.org/10.1155/2016/3419479

Kocaman N, Artas G. Can novel adipokines, asprosin and meteorin-like, be biomarkers for malignant mesothelioma? Biotech Histochem. 2020;95(3):171-5. doi: 10.1080/10520295.2019.1656344. DOI: https://doi.org/10.1080/10520295.2019.1656344

Erman H, Böyük B, Cetın SI, Sevınc S, Bulut U, Maviş O. Beta cell function as an assessment tool for cardiovascular risk in patients with metabolic syndrome. J Surg Med. 2021;5(10):1002-6. doi: 10.28982/josam.847350 DOI: https://doi.org/10.28982/josam.847350

Kolodziejski PA, Leciejewska N, Chmurzynska A, Sassek M, Szczepankiewicz A, Szczepankiewicz D, et al. 30-Day spexin treatment of mice with diet-induced obesity (DIO) and type 2 diabetes (T2DM) increases insulin sensitivity, improves liver functions and metabolic status. Mol Cell Endocrinol. 2021;536:111420. doi: 10.1016/j.mce.2021.111420. DOI: https://doi.org/10.1016/j.mce.2021.111420

Ge JF, Walewski JL, Anglade D, Berk PD. Regulation of hepatocellular fatty acid uptake in mouse models of fatty liver disease with and without functional leptin signaling: roles of NfKB and SREBP-1C and the effects of spexin. Semin Liver Dis. 2016;36(4):360-72. doi: 10.1055/s-0036-1597248. DOI: https://doi.org/10.1055/s-0036-1597248

Walewski JL, Ge F, Lobdell H 4th, Levin N, Schwartz GJ, Vasselli JR, et al. Spexin is a novel human peptide that reduces adipocyte uptake of long chain fatty acids and causes weight loss in rodents with diet-induced obesity. Obesity (Silver Spring). 2014;22(7):1643-52. doi: 10.1002/oby.20725. DOI: https://doi.org/10.1002/oby.20725

Behrooz M, Vaghef-Mehrabany E, Ostadrahimi A. Different spexin level in obese vs normal weight children and its relationship with obesity related risk factors. Nutr Metab Cardiovasc Dis. 2020;30(4):674-82. doi: 10.1016/j.numecd.2019.11.008. DOI: https://doi.org/10.1016/j.numecd.2019.11.008

Bali EB, Ergin V, Rackova L, Bayraktar O, Küçükboyaci N, Karasu Ç. Olive leaf extracts protect cardiomyocytes against 4-hydroxynonenal-induced toxicity in vitro: Comparison with oleuropein, Hydroxytyrosol, and quercetin. Planta Med. 2014;80(12):984-92. doi: 10.1055/s-0034-1382881. DOI: https://doi.org/10.1055/s-0034-1382881

Mnafgui K, Hajji R, Derbali F, Khlif I, Kraiem F, Ellefi H, et al. Protective Effect of Hydroxytyrosol Against Cardiac Remodeling After Isoproterenol-Induced Myocardial Infarction in Rat. Cardiovasc Toxicol. 2016;16(2):147-55. doi: 10.1007/s12012-015-9323-1. DOI: https://doi.org/10.1007/s12012-015-9323-1

Cao K, Xu J, Zou X, Li Y, Chen C, Zheng A, et al. Hydroxytyrosol prevents diet-induced metabolic syndrome and attenuates mitochondrial abnormalities in obese mice. Free Radic Biol Med. 2014;67:396-407. doi: 10.1016/j.freeradbiomed.2013.11.029. DOI: https://doi.org/10.1016/j.freeradbiomed.2013.11.029

Scoditti E, Nestola A, Massaro M, Calabriso N, Storelli C, Caterina RD, et al. Hydroxytyrosol suppresses MMP-9 and COX-2 activity and expression in activated human monocytes via PKCα and PKCβ1 inhibition. Atherosclerosis. 2014;232(1):17-24. doi: 10.1016/j.atherosclerosis.2013.10.017. DOI: https://doi.org/10.1016/j.atherosclerosis.2013.10.017

Garlanda C, Bottazzi B, Bastone A, Mantovani A. Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility. Annu Rev Immunol. 2005;23:337-66. doi: 10.1146/annurev.immunol.23.021704.115756. DOI: https://doi.org/10.1146/annurev.immunol.23.021704.115756

Muller B, Peri G, Doni A, Torri V, Landmann R, Bottazzi B, et al. Circulating levels of the long Pentraxin PTX3 correlate with severity of infection in critically ill patients. Crit Care Med. 2001;29(7):1404-7. doi: 10.1097/00003246-200107000-00017. DOI: https://doi.org/10.1097/00003246-200107000-00017

Ohbayashi H, Miyazawa C, Miyamoto K, Sagara M, Yamashita T, Onda R. Pitavastatin improves plasma Pentraxin 3 and arterial stiffness in atherosclerotic patients with hypercholesterolemia. J Atheroscler Thromb. 2009;16(4):490-500. doi: 10.5551/jat.no613. DOI: https://doi.org/10.5551/jat.No613

Barbier P, Alioto G, Guazzi MD. Left atrial function and ventricular filling in hypertensive patients with paroxysmal atrial fibrillation. J Am Coll Cardiol. 1994;24(1):165-70. doi: 10.1016/0735-1097(94)90558-4. DOI: https://doi.org/10.1016/0735-1097(94)90558-4

Zanetti M, Bosutti A, Ferreira C, Vinci P, Biolo G, Fonda M, et al. Circulating Pentraxin 3 levels are elevated in metabolic syndrome with subclinical atherosclerosis: Evidence for association with atherogenic lipid profile. Clin Exp Med. 2009;9(3):243-8. doi: 10.1007/s10238-009-0039-z. DOI: https://doi.org/10.1007/s10238-009-0039-z

Karakas MF, Buyukkaya E, Kurt M, Motor S, Akcay AB, Karakas E, et al. Serum Pentraxin-3 levels are associated with the severity of metabolic syndrome. Med Princ Pract. 2013;22(3):274-9. doi: 10.1159/000343904. DOI: https://doi.org/10.1159/000343904

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Published

2023-09-29

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1.
Onat E, Türk A. The protective effect of hydroxytyrosol on the heart in rats fed corn syrup: The role of spexin, pentraxin-3 : The protective effect of hydroxytyrosol on heart. J Surg Med [Internet]. 2023 Sep. 29 [cited 2024 Jun. 14];7(9):632-6. Available from: https://jsurgmed.com/article/view/7940