Does flaxseed and chia use affect postprandial glucose, insulin and subjective saturation response in healthy individuals?

Effect of chia and flaxseed on glycemic control and satiety



Chia, Flaxseed, Blood Glucose, Insulin, Appetite


Background/Aim: In recent years, there has been an increase in the prevalence of obesity and its complications, along with a growing awareness of healthy nutrition. As a result, consumers are seeking to incorporate more functional foods into their diets. Chia and flax seeds have gained popularity due to their soluble fiber and antioxidant capacity. This study aims to compare the effects of consuming cakes made with the addition of chia and flax seeds on blood glucose and insulin levels, as well as evaluate their impact on post-consumption satiety response in individuals.

Methods: This randomized, double-blind, self-controlled experimental study involved 12 volunteers (19–64 years old) who were free from acute or chronic diseases. The participants had a body mass index (BMI) value between 18.5 and 24.9 kg/m2 and a Beck Depression Inventory score of 8 or below. The study investigated the effects of standard and test cakes containing 50 g of digestible carbohydrates, including chia-added cake, flaxseed-added cake, and chia+flaxseed-added cake. Postprandial blood sugar, insulin, and subjective satiety responses were assessed. A standard nutrition program (diet: 60% carbohydrates, 20% protein, 30% fat) was implemented at least one week before the study, and participants were asked to maintain 24-h food consumption records the day before the test days. Throughout the study period, individuals were instructed to avoid caffeine, medication, nutritional supplements, and heavy physical activity. Cake consumption sessions were conducted at the research center, with participants visiting four times in total, with at least 1-week intervals. Fasting for 10–12 h prior to each visit, saturation responses were measured using a visual analog scale at 0, 15, 30, 60, 90, 120, and 180 min. Blood samples were also collected to assess blood glucose and insulin levels.

Results: The study revealed that cakes containing chia and flaxseeds, compared to the standard cake, as well as flaxseed-added cake compared to chia-added cake, resulted in higher plasma glucose under-curve values and saturation responses and lower hunger responses (P=0.038, P=0.016, P=0.004, respectively).

Conclusion: The findings indicate that both chia and flax seeds impact glycemic control and the sensation of satiety, with flaxseed exhibiting greater effectiveness than chia.


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Green M, Arora K, Prakash S. Microbial medicine: prebiotic and probiotic functional foods to target obesity and metabolic syndrome. Int J Mol Sci. 2020;21(8):2890. doi: 10.3390/ijms21082890. DOI:

Mohd Ali N, Yeap SK, Ho WY, Beh BK, Tan SW, Tan SG. The Promising future of chia, Salvia hispanica L. J Biomed Biotechnol. 2012;2012:171956. doi: 10.1155/2012/171956 . DOI:

Carraro JCC, Dantas MS, Espeschit ACR, Martino HSD, Ribeiro SMR. Flaxseed and human health: reviewing benefits and adverse effects. Food Reviews International. 2012;28(2):203-30. doi: 10.1080/87559129.2011.595025. DOI:

Unites States Department of Agriculture Research Service. USDA Food Composition Databese. [Cited 2022/5/8]; Available from:

Ramcharitar A, Badrie N, Mattfeldt‐Beman M, Matsuo H, Ridley C. Consumer acceptability of muffins with flaxseed (Linum usitatissimum). Journal of Food Science. 2005;70(7):504-7. doi: 10.1111/j.1365-2621.2005.tb11499.x. DOI:

Rashed MM, Shallan M, Mohamed D, Fouda K, Hanna L. Biological evaluation of anti-androgenic effect of some plant foods. J Food Nutr Res. 2014;2(9):645-51. doi: 10.12691/jfnr-2-9-19. DOI:

Malkki Y. Physical properties of dietary fiber as keys to physiological functions. Cereal Foods World. 2001;46(5):196-9.

Klotzbach-Shimomura K. Functional foods: The role of physiologically active compounds in relation to disease. Topics in Clinical Nutrition. 2001;16(2):68-78. DOI:

Marcinek K, Krejpcio Z. Chia seeds (Salvia hispanica): health promoting properties and therapeutic applications – a review. Rocz Panstw Zakl Hig. 2017;68(2):123-9.

Pan A, Yu D, Demark-Wahnefried W, Franco O, Lin X. Meta-analysis of the effects of flaxseed interventions on blood lipids. Am J Clin Nutr. 2009;90(2):288-97. doi: 10.3945/ajcn.2009.27469. DOI:

Nieman DC, Cayea EJ, Austin MD, Henson DA, McAnulty SR, Jin F. Chia seed does not promote weight loss or alter disease risk factors in overweight adults. Nutr Res. 2009;29(6):414-8. doi: 10.1016/j.nutres.2009.05.011. DOI:

Vuksan V, Jenkins AL, Brissette C, Choleva L, Jovanovski E, Gibbs AL, et al. Salba-chia (Salvia hispanica L.) in the treatment of overweight and obese patients with type 2 diabetes: A double-blind randomized controlled trial. Nutr Metab Cardiovasc Dis. 2017;27(2):138-46. doi: 10.1016/j.numecd.2016.11.124. DOI:

Vuksan V, Choleva L, Jovanovski E, Jenkins AL, Au-Yeung F, Dias AG, et al. Comparison of flax (Linum usitatissimum) and Salba-chia (Salvia hispanica L.) seeds on postprandial glycemia and satiety in healthy individuals: a randomized, controlled, crossover study. Eur J Clin Nutr. 2017;71(2):234-8. doi: 10.1038/ejcn.2016.148. DOI:

Mani UV, Mani I, Biswas M, Kumar SN. An open-label study on the effect of flax seed powder (Linum usitatissimum) supplementation in the management of diabetes mellitus. J Diet Suppl. 2011;8(3):257-65. doi: 10.3109/19390211.2011.593615. DOI:

Hutchins AM, Brown BD, Cunnane SC, Domitrovich SG, Adams ER, Bobowiec CE. Daily flaxseed consumption improves glycemic control in obese men and women with pre-diabetes: a randomized study. Nutr Res. 2013;33(5):367-75. doi: 10.1016/j.nutres.2013.02.012. DOI:

Schmind M. Beslenme Bilgi Sistemi BEBİS 8.2 Paket programı Entwickelt an der Universital Hohenheim, Struttgart, Germany. 2011. Available at:

AACC. International Approved Methods of Analysis, American Association of Cereal Chemists (11 th Edition), USA: St. Paul MN; 2010.

A.O.A.C. Official methods of analysis (15 th Edition), USA: Arlington VA; 1990.

Anonim. T.C. Gıda, Tarım ve Hayvancılık Bakanlığı, Ulusal Gıda Kompozisyonu Veri Tabanı (Türkomp); 2018.

A.O.A.C. Official Methods for Analysis, Association of Official Analytical Chemists (17 th Edition), USA: Arlington VA; 2000.

A.A.C.C. American Association of Cereal Chemists International, Approved Methods of the AACC the Association (8 th Edition), USA: St. Paul MN; 2000.

A.O.A.C Official Method 991.43. Total, soluble and insoluble dietary fiber in foods. In: Official methods of analysis of AOAC international. 16 th Ed. Cunniff P, eds. Gaithersburg, AOAC International Chapter 32; 1999. pp. 7-9.

Flint A, Raben A, Blundell JE, Astrup A. Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes Relat Metab Disord. 2000;24(1):38-48. doi: 10.1038/sj.ijo.0801083. DOI:

Shaikh RP, Gadhe K. Studies on development and quality evaluation of cupcake fortified with flaxseed and chia seed flour. Pharma Innov. 2020;9:214-7.

Lipilina E, Ganji V. Incorporation of ground flaxseed into bakery products and its effect on sensory and nutritional characteristics – a pilot study. Journal of Foodservice. 2009;20(1):52-9. doi: 10.1111/j.1748-0159.2008.00124.x. DOI:

Chelladurai C, Pandey A, Panmand SA, Nikam S. Development of innovative bakery product chia seed enriched cookies. Int J Food Sci Nutr. 2019;4:19-23.

Steffoloni E, De La Hera E, Perez G, Gomez M. Effect of chia (Salvia Hispanica L) addition on the quality of gluten-free bread. Journal of Food Quality. 2014;37(5):309-17. doi: 10.1111/jfq.12098. DOI:

Pohjanheimo T, Hakala M, Tahvonen R, Salminen S, Kallio H. Flaxseed in breadmaking: effects on sensory quality, aging, and composition of bakery products. Journal of Food Science. 2006;71(4):343-8. doi: 10.1111/j.1750-3841.2006.00005.x. DOI:

Dündar AN, Aydın E, Yıldız E, Parlak O. Effects of chia seed properties and quality characteristics of regular and low-fat crackers. Food Science and Technology. 2021;41:4. doi: 10.1590/fst.26120. DOI:

Zhu F, Chan C. Effect of chia seed on glycemic response, texture, and sensory properties of Chinese steamed bread. LWT. 2018;98:77-84. doi: 10.1016/J.LWT.2018.08.016. DOI:

Koca A, Anil M. Effect of flaxseed and wheat flour blends on dough rheology and bread quality. J Sci Food Agric. 2007;87(6):1172-5. doi: 10.1002/jsfa.2739. DOI:

Merenkova SP, Lukin AA, Klejman DA. Influence of flaxseed flour additives on quality parameters of bakery products. News of institutes of higher education Food Technology. 2016;5;10-3.

R Core Team. R: A language and environment for statistical computing. In: Computing. RFfS, editor. Vienna, Austria. 2019. Available at:

Au MMC, Goff H, Kisch J, Coulson A, Wright A. Effects of soy-soluble fiber and flaxseed gum on the glycemic and insulinemic responses to glucose solutions and dairy products in healthy adult males. J Am Coll Nutr. 2013;32(2):98-100. doi: 10.1080/07315724.2013.767579. DOI:

Pan A, Sun J, Chen Y, et al. Effects of a flaxseed-derived lignan supplement in type 2 diabetic patients: a randomized, double-blind, cross-over trial. PLoS One. 2007;2(11):1148. doi: 10.1371/journal.pone.0001148. DOI:

Karakas SE, Perroud B, Kind T, Palazoglu M, Fiehn O. Changes in plasma metabolites and glucose homeostasis during omega-3 polyunsaturated fatty acid supplementation in women with polycystic ovary syndrome. BBA Clin. 2016;5:179-85. doi: 10.1016/j.bbacli.2016.04.003. DOI:

Nettleton JA, Katz R. n-3 long-chain polyunsaturated fatty acids in type 2 diabetes: a review. J Am Diet Assoc. 2005;105(3):428-40. doi: 10.1016/j.jada.2004.11.029. DOI:

Machado AM, de Paula H, Cardoso LD, Costa NMB. Effects of brown and golden flaxseed on the lipid profile, glycemia, inflammatory biomarkers, blood pressure and body composition in overweight adolescents. Nutrition. 2015;31(1):90-6. doi: 10.1016/j.nut.2014.05.002. DOI:

Stuglin C, Prasad K. Effect of flaxseed consumption on blood pressure, serum lipids, hemopoietic system and liver and kidney enzymes in healthy humans. J Cardiovasc Pharmacol Ther. 2005;10(1):23-7. doi: 10.1177/107424840501000103. DOI:

Dahl WJ, Lockert EA, Cammer AL, Whiting SJ. Effects of flax fiber on laxation and glycemic response in healthy volunteers. J Med Food. 2005;8(4):508-11. doi: 10.1089/jmf.2005.8.508. DOI:

Barre DE, Mizier-Barre KA, Griscti O, Hafez K. High dose flaxseed oil supplementation may affect fasting blood serum glucose management in human type 2 diabetics. J Oleo Sci. 2008;57(5):269-73. doi: 10.5650/jos.57.269. DOI:

Cornish SM, Chilibeck PD, Paus-Jennsen L, Biem HJ, Khozani T, Senanayake V, et al. A randomized controlled trial of the effects of flaxseed lignan complex on metabolic syndrome composite score and bone mineral in older adults. Appl Physiol Nutr Metab. 2009;34(2):89-98. doi:10.1139/H08-142. DOI:

Bernacchia R, Preti R, Vinci G. Chemical composition and health benefits of flaxseed. Austin J Nutr Food Sci. 2014;2(8):1045.

Coorey R, Tjoe A, Jayasena V. Gelling properties of chia seed and flour. J Food Sci. 2014;79(5):859-66. doi: 10.1111/1750-3841.12444. DOI:

da Silva BP, Anunciação PC, Matyelka JCDS, Della Lucia CM, Martino HSD, Pinheiro-Sant’Ana HM. Chemical composition of Brazilian chia seeds grown in different places. Food Chemistry. 2017;221:1709-16. doi: 10.1016/j.foodchem.2016.10.115. DOI:

Morisset AS, Lemieux S, Veilleux A, Bergeron J, John Weisnagel S, Tchernof A. Impact of a lignan-rich diet on adiposity and insulin sensitivity in post-menopausal women. Br J Nutr. 2009;102(2):195-200. doi: 10.1017/s0007114508162092. DOI:

Clarke ST, Sarfaraz S, Qi X, Ramdath DG, Fougere GC, Ramdath DD. A Review of the Relationship between lentil serving and acute postprandial blood glucose response: effects of dietary fibre, protein and carbohydrates. Nutrients. 2022;14(4);849. doi: 10.3390/nu14040849. DOI:

Mohammadi-Sartang M, Sohrabi Z, Barati-Boldaji R, Raeisi-Dehkordi H, Mazloom Z. Flaxseed supplementation on glucose control and insulin sensitivity: a systematic review and meta-analysis of 25 randomized, placebo-controlled trials. Nutr Rev. 2018;76(2):125-39. doi: 10.1093/nutrit/nux052. DOI:

Khalesi S, Irwin C, Schubert M. Flaxseed consumption may reduce blood pressure: a systematic review and meta-analysis of controlled trials. J Nutr. 2015;145(4):758-65. doi: 10.3945/jn.114.205302. DOI:

Albert BB, Derraik JGB, Brennan CM, Biggs JB, Smith GC, Garg ML, et al. Higher omega-3 index is associated with increased insulin sensitivity and more favourable metabolic profile in middle-aged overweight men. Sci Rep. 2014;4:6697. doi: 10.1038/srep06697. DOI:

Wien M, Rajaram S, Oda K, Sabaté J. Decreasing the linoleic acid to alpha-linolenic acid diet ratio increases eicosapentaenoic acid in erythrocytes in adults. Lipids. 2010;45(8):683-92. doi: 10.1007/s11745-010-3430-3. DOI:

Karlsson FHh, Tremaroli V, Nookaew I, Bergström G, Behre CJ, Fagerberg BF, et al. Gut metagenome in European women with normal, impaired and diabetic glucose control. Nature. 2013;498(7452):99-103. doi: 10.1038/nature12198. DOI:

Kuo SM. The interplay between fiber and the intestinal microbiome in the inflammatory response. Adv Nutr. 2013;4(1):16-28. doi: 10.3945/an.112.003046. DOI:

Hervik AK, Svihus B. The role of fiber in energy balance. J Nutr Metab. 2019;2019:4983657. doi: 10.1155/2019/4983657. DOI:

Tremblay A, Bellisle F. Nutrients, satiety, and control of energy intake. Appl Physiol Nutr Metab. 2015;40(10):971-9. doi: 10.1139/apnm-2014-0549. DOI:

Capuano E. The behavior of dietary fiber in the gastrointestinal tract determines its physiological effect. Crit Rev Food Sci Nutr. 2017;57(16):3543-64. doi: 10.1080/10408398.2016.1180501. DOI:

Ho IH, Matia-Merino L, Huffman LM. Use of viscous fibres in beverages for appetite control: a review of studies. Int J Food Sci Nutr. 2015;66(5):479-90. doi: 10.3109/09637486.2015.1034252. DOI:

Sánchez D, Miguel M, Aleixandre A. Dietary fiber, gut peptides, and adipocytokines. J Med Food. 2012;15(3):223-30. doi: 10.1089/jmf.2011.0072. DOI:

Rahman S, Zhao A, Xiao D, Park E, Edirisinghe I, Burton-Freeman B. A randomized, controlled trial evaluating polydextrose as a fiber in a wet and dry matrix on glycemic control. J Food Sci. 2017;82(10):2471-8. doi: 10.1111/1750-3841.13855. DOI:

Tarini J, Wolever TM. The fermentable fibre inulin increases postprandial serum short-chain fatty acids and reduces free-fatty acids and ghrelin in healthy subjects. Appl Physiol Nutr Metab. 2010;35(1):9-16. doi: 10.1139/H09-119. DOI:

Ye Z, Arumugam V, Haugabrooks E, Williamson P, Hendrich S. Soluble dietary fiber (Fibersol-2) decreased hunger and increased satiety hormones in humans when ingested with a meal. Nutr Res. 2015;35(5):393-400. doi: 10.1016/j.nutres.2015.03.004. DOI:

Lumaga RB, Azzali D, Fogliano V, Scalfi L, Vitaglione P. Sugar and dietary fibre composition influence, by different hormonal response, the satiating capacity of a fruit-based and a β-glucan-enriched beverage. Food Funct. 2012;3(1):67-75. doi: 10.1039/c1fo10065c. DOI:

Rahat-Rozenbloom S, Fernandes J, Cheng J, Wolever TMS. Acute increases in serum colonic short-chain fatty acids elicited by inulin do not increase GLP-1 or PYY responses but may reduce ghrelin in lean and overweight humans. Eur J Clin Nutr. 2017;71(8):953-8. doi: 10.1038/ejcn.2016.249. DOI:

Cohen L, Meira J, Cosendey GM, de Souza AF, Mattos F, Carneiro JRI, et al. Evaluation of the influence of whole and defatted flaxseed on satiety, glucose, and leptin levels of women in the late postoperative stage of bariatric surgery. Obes Surg. 2013;23(2):157-66. doi: 10.1007/s11695-012-0733-x. DOI:

Lafond DW, Greaves KA, Maki KC, Leidy HJ, Romsos DR. Effects of two dietary fibers as part of ready-to-eat cereal (RTEC) breakfasts on perceived appetite and gut hormones in overweight women. Nutrients. 2015;7(2):1245-66. doi: 10.3390/nu7021245. DOI:

Vuksan V, Jenkins AL, Dias AG, Lee AS, Jovanovski E, Rogovik AL, et al. Reduction in postprandial glucose excursion and prolongation of satiety: possible explanation of the long-term effects of whole grain Salba (Salvia Hispanica L.). Eur J Clin Nutr. 2010;64(4):436-8. doi: 10.1038/ejcn.2009.159. DOI:

Ibrügger S, Kristensen M, Mikkelsen MS, Astrup A. Flaxseed dietary fiber supplements for suppression of appetite and food intake. Appetite. 2012;58(2):490-5. doi: 10.1016/j.appet.2011.12.024. DOI:

Reimer RA, Pelletier X, Carabin IG, Lyon M, Gahler R, Parnell JA, et al. Increased plasma PYY levels following supplementation with the functional fiber PolyGlycopleX in healthy adults. Eur J Clin Nutr. 2010;64(10):1186-91. doi:10.1038/ejcn.2010.141. DOI:

Sandberg JC, Björck IME, Nilsson AC. Effects of whole grain rye, with and without resistant starch type 2 supplementation, on glucose tolerance, gut hormones, inflammation and appetite regulation in an 11-14.5 hour perspective; a randomized controlled study in healthy subjects. Nutr J. 2017;16:25. doi: 10.1186/s12937-017-0246-5. DOI:

Juvonen KR, Salmenkallio-Marttila M, Lyly M, Liukkonen KH, Lahteenmaki L, Laaksonen DE, et al. Semisolid meal enriched in oat bran decreases plasma glucose and insulin levels, but does not change gastrointestinal peptide responses or short-term appetite in healthy subjects. Nutr Metab Cardiovasc Dis. 2011;21(9):748-56. doi: 10.1016/j.numecd.2010.02.002. DOI:

Karhunen LJ, Juvonen KR, Flander SM, Luikkonen KH, Lahteenmaki L, Siloaho M, et al. A psyllium fiber-enriched meal strongly attenuates postprandial gastrointestinal peptide release in healthy young adults. J Nutr. 2010;140(4):737-44. doi: 10.3945/jn.109.115436. DOI:






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Özpak Akkuş Özlem, Özdemir Y, Özbek Çağla, Öncel B, Yapıcı Nane B. Does flaxseed and chia use affect postprandial glucose, insulin and subjective saturation response in healthy individuals? Effect of chia and flaxseed on glycemic control and satiety. J Surg Med [Internet]. 2023 Jul. 21 [cited 2023 Sep. 26];7(8):434-40. Available from: