Astaxanthin and Coenzyme Q10 are not synergistic against oxidative damage in cerulein-induced acute pancreatitis

Esra Bihter Gürler; Dilek Özbeyli; Hülya Buzcu; Muhammet Emin Çam; Meral Yüksel

doi:10.28982/josam.756220

Authors

Esra Bihter Gürler https://orcid.org/0000-0001-8259-521X
Dilek Özbeyli https://orcid.org/0000-0002-4141-6913
Hülya Buzcu https://orcid.org/0000-0001-9379-6340
Muhammet Emin Çam https://orcid.org/0000-0001-5398-6801
Meral Yüksel https://orcid.org/0000-0002-4760-3306

DOI:

https://doi.org/10.28982/josam.756220

Keywords:

Astaxanthin, Coenzyme Q10, Oxidative Damage, Acute pancreatitis

Abstract

Background/Aim: The anti-inflammatory effects of Astaxanthin (ATX) and Coenzyme Q10 (Ubiquinone) are studied in different inflammation models. The study aims to investigate the synergistic effect of astaxanthin and Coenzyme Q10 in a rat model of acute pancreatitis. Methods: Twenty-four female Wistar rats were grouped as control (C), carrier-treated control (AP), astaxanthin-treated (ATX; 40 mg/kg), astaxanthin+coenzyme Q10- treated (ATX+Q10; 40 mg/kg and 1 gr/kg) groups. Cerulein was administered (50 µg/kg) twice, one hour apart. Seven hours after the first cerulein injection, the rats were sacrificed. Pancreatic oxidative damage was evaluated with an increase in the serum activity of lipase and amylase, tissue levels of myeloperoxidase activity (MPO), malondialdehyde (MDA), luminol and lucigenin and decrease of glutathione. Results: In all AP groups, MDA and MPO increased while GSH decreased (P<0.001). ATX and ATX+Q10 both decreased MDA and MPO (P<0.001) and increased GSH (P<0.01). Both therapies significantly increased luminol levels (P<0.001, and P<0.01, respectively). Lucigenin markedly decreased in the ATX+Q10 group (P<0.01). Conclusion: Antioxidant combination therapy does not alleviate oxidative damage in pancreatic tissue better than astaxanthin alone.

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