Investigation of the relationship between serum adropin levels, oxidative stress biomarkers, and blood pressure in DOCA-salt hypertensive rats: DOCA-salt hypertension and adropin

Raziye  Akcilar

doi:10.28982/josam.1061010

Authors

Raziye Akcilar Department of Physiology, Faculty of Medicine, Kütahya Health Sciences University, Kütahya, Turkey https://orcid.org/0000-0003-4720-1945

DOI:

https://doi.org/10.28982/josam.1061010

Keywords:

Adropin, Oxidative stress, DOCA-salt hypertension, Blood pressure

Abstract

Background/Aim: Adropin is involved in the pathophysiology and development of cardiovascular diseases, such as hypertension. The aim of this study was to investigate the effects of adropin in serum, potential use as a biochemical biomarker of oxidative stress, and effects on blood pressure in deoxycorticosterone acetate (DOCA) salt hypertensive rats.

Methods: Eighteen male Sprague-Dawley rats were divided into two groups: (1) Control (C) and (2) Hypertensive (H). Systolic and diastolic blood pressures (SBP and DBP, respectively), and mean blood pressure (MBP) were measured using the tail-cuff method. At the end of the study, serum endothelin-1 (ET-1), adropin, nitric oxide (NO), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were also analyzed.

Results: Significant increases in SBP, DBP, MBP, cardiac hypertrophy index (CHI), and left ventricular hypertrophy index (LVCI) in the H group compared with the C group were found. Serum levels of ET-1, TOS, and OSI were significantly higher in the H group and serum levels of NO, adropin, and TAS were lower than in the C group. A negative correlation between serum adropin levels and the variables SBP, DBP, MBP, TOS, OSI, CHI, and LVHI was found. Adropin levels were positively correlated positively with serum NO levels in both groups.

Conclusion: Serum adropin levels decreased in hypertensive DOCA-salt rats. Lower serum adropin levels were found to be significantly associated with hypertension and may play a role in this disease. However, further comprehensive and diverse studies are needed.

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References

Aydin S, Kuloglu T, Aydin S, Eren MN, Yilmaz M, Kalayci M, et al. Expression of adropin in rat brain, cerebellum, kidneys, heart, liver, and pancreas in streptozotocin-induced diabetes. Mol Cell Biochem. 2013;380(1-2):73-81. DOI: https://doi.org/10.1007/s11010-013-1660-4

Topuz M, Celik A, Aslantas T, Demir AK, Aydin S, Aydin S. Plasma adropin levels predict endothelial dysfunction like flow-mediated dilatation in patients with type 2 diabetes mellitus. J Investig Med. 2013;61:1161-4. DOI: https://doi.org/10.2310/JIM.0000000000000003

Aydin H, Türk A, Karadağ A. Effects of vitamin D treatment on the heart tissue and adropin levels in thyrotoxicosis rats. J Surg Med. 2022;6(3):382-385. DOI: https://doi.org/10.28982/josam.961385

Coffman TM. Under pressure: the search for the essential mechanisms of hypertension. Nature Medicine. 2011;17(11):1402-9. DOI: https://doi.org/10.1038/nm.2541

Gomez-Sanchez EP. DOCA/Salt: Much More Than a Model of Hypertension. J Cardiovasc Pharmacol. 2019;74(5):369-71. DOI: https://doi.org/10.1097/FJC.0000000000000753

Lee LK, Kim MY, Kim JH, Lee JU, Park BS, Yang SM, et al. A review of deoxycorticosterone acetate-salt hypertension and its relevance for cardiovascular physiotherapy research. J Phys Ther Sci. 2015;27(1):303-7. DOI: https://doi.org/10.1589/jpts.27.303

Bhatia J, Tabassum F, Sharma AK, Bharti S, Golechha M, Joshi S, et al. Emblica officinalis exerts antihypertensive effect in a rat model of DOCA-salt-induced hypertension: role of (p) eNOS, NO and oxidative stress. Cardiovasc Toxicol. 2011;11(3):272-9. DOI: https://doi.org/10.1007/s12012-011-9122-2

Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004;37:277-85. DOI: https://doi.org/10.1016/j.clinbiochem.2003.11.015

Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38:1103-11. DOI: https://doi.org/10.1016/j.clinbiochem.2005.08.008

Hebden RA, Todd ME, Tang C, Gowen B, McNeill JH. Association of DOCA hypertension with induction of atherosclerosis in rats with short-term diabetes mellitus. Am J Physiol. 1990;258:RI042-R1050. DOI: https://doi.org/10.1152/ajpregu.1990.258.4.R1042

Akcılar R, Akçer S, Şimşek H, Akcılar A, Bayat Z, Genç O. The effect of ozone on blood pressure in DOCA-salt-induced hypertensive rats. Int J Clin Exp Med. 2015;15;8(8):12783-91.

Akcılar R, Turgut S, Caner V, Akcılar A, Ayada C, Elmas L, et al. Apelin effects on blood pressure and RAS in DOCA-salt-induced hypertensive rats. Clin Exp Hypertens. 2013;35(7):550-7. DOI: https://doi.org/10.3109/10641963.2013.764889

Schiffrin EL. Role of Endothelin-1 in hypertension and vascular disease. Am J Hypertens. 2001;14:83-89. DOI: https://doi.org/10.1016/S0895-7061(01)02074-X

Basting T, Lazartigues E. DOCA-Salt Hypertension: an Update. Curr Hypertens Rep. 2017;19(4):32. DOI: https://doi.org/10.1007/s11906-017-0731-4

Cheng WH, Lu PJ, Hsiao M, Hsiao CH, Ho WY, Cheng PW, et al. Renin activates PI3K-Akt-eNOS signalling through the angiotensin AT₁ and Mas receptors to modulate central blood pressure control in the nucleus tractus solitarii. Br J Pharmacol. 2012;166(7):2024-35. DOI: https://doi.org/10.1111/j.1476-5381.2012.01832.x

Sato A, Nishioka S, Kiuchi M, Imada Y, Makino K, Nakagawa K, et al. Grape Extract from Chardonnay Seeds Restores Deoxycorticosterone Acetate-Salt-Induced Endothelial Dysfunction and Hypertension in Rats. Biol Pharm Bull. 2020;43(1):59-67. DOI: https://doi.org/10.1248/bpb.b19-00540

Lovren F, Pan Y, Quan A, Singh KK, Shukla PC, Gupta M, et al. Adropin is a novel regulator of endothelial function. Circulation. 2010;14(1):S185-92. DOI: https://doi.org/10.1161/CIRCULATIONAHA.109.931782

Allcock GH, Hukkanen M, Polak JM, Pollock JS, Pollock DM. Increased nitric oxide synthase-3 expression in kidneys of deoxycorticosterone acetate-salt hypertensive rats. J Am Soc Nephrol. 1999;10:2283-9. DOI: https://doi.org/10.1681/ASN.V10112283

Han S, Bal NB, Sadi G, Usanmaz SE, Tuglu MM, Uludag MO, et al. Inhibition of endoplasmic reticulum stress protected DOCA-salt hypertension-induced vascular dysfunction. Vascul Pharmacol. 2019;113:38-46. DOI: https://doi.org/10.1016/j.vph.2018.11.004

Gulen B, Eken C, Kucukdagli OT, Serinken M, Kocyigit A, Kılıc E, et al. Adropin levels and target organ damage secondary to high blood pressure in the ED. Am J Emerg Med. 2016;34(11):2061-4. DOI: https://doi.org/10.1016/j.ajem.2016.04.014

Bolayır HA, Kıvrak T, Gunes H, Bolayır A, Karaca I. Adropin and circadian variation of blood pressure. Kardiol Pol. 2018;76(4):776-82. DOI: https://doi.org/10.5603/KP.2018.0006

Gu X, Li H, Zhu X, Gu H, Chen J, Wang L, et al. Inverse Correlation Between Plasma Adropin and ET-1 Levels in Essential Hypertension: A Cross-Sectional Study. Medicine (Baltimore). 2015;94(40):e1712. DOI: https://doi.org/10.1097/MD.0000000000001712

Altincik A, Sayin O. Evaluation of the relationship between serum adropin levels and blood pressure in obese children. J Pediatr Endocrinol Metab. 2015;28(9-10):1095-100. DOI: https://doi.org/10.1515/jpem-2015-0051

Zhang Y, Ji Z, Yuan JY, Wang ZJ, Liang JR, Wu JJ, et al. Regulating effect of electroacupuncture on left ventricular function and autophagy-specific proteins in spontaneously hypertension rats. Zhen Ci Yan Jiu. 2019;25;44(2):90-4.

Ozdemir B, Gulhan MF, Sahna E, Selamoglu Z. The investigation of antioxidant and anti-inflammatory potentials of apitherapeutic agents on heart tissues in nitric oxide synthase inhibited rats via Nomega-nitro-L-arginine methyl ester. Clin Exp Hypertens. 2021;2;43(1):69-76. DOI: https://doi.org/10.1080/10641963.2020.1806294

Gulhan MF. Therapeutic potentials of propolis and pollen on biochemical changes in reproductive function of L-NAME induced hypertensive male rats. Clin Exp Hypertens. 2019;41(3):292-8. DOI: https://doi.org/10.1080/10641963.2018.1506470