Effects of Algan hemostatic agent foam in rat femoral artery injury model: A randomized animal trial

Algan hemostatic agent act on artery injury model

Authors

Keywords:

Algan hemostatic agent, Femoral artery, Hemostasis, Bleeding, Rat, Coagulation

Abstract

Background/Aim: Nowadays, many deaths are related to vessel injury-induced blood loss. Failure to control bleeding also increases the risk of death. This study aimed to investigate the hemostatic effects of the Algan Hemostatic Agent (AHA) foam application in a rat model in which severe femoral artery bleeding was induced.

Methods: Fourteen rats were randomly assigned to two groups: (1) control (physiological saline) (n = 7) and (2) AHA foam (n = 7). The left femoral artery of the rats was incised and when the bleeding started, and the area was pressed with another sponge for 10 s in all rats. Afterwards, physiological saline solution impregnated gauze or AHA foam was placed over same area. A chronometer was started and area was checked after 2 min. If no bleeding occurred during the first 2 min of application, it was recorded as “successful”. If bleeding occurred, the same procedure was repeated up to three times. If hemostasis could not be achieved even after the third application, it was considered a failure, and “failed” was recorded. All animals were sacrificed under high anesthesia for least 10 min after the experiment.

Results: Application of AHA resulted in complete (100%) control of bleeding in all rats within the first 2 min. In control group, hemostasis was achieved in 1 out of 7 (14.3%) rats by the third application. Failure was recorded for the remaining six rats. The hemostatic success rate of the AHA foam was significantly higher than the rates of control group (P = 0.005).

Conclusion: AHA foam is a very effective hemostatic agent and can be applied easily on vascular trauma models. Further studies are needed to elucidate hemostatic features of AHA.

Downloads

Download data is not yet available.

References

Cameron PA, Knapp BJ, Teeter W. Trauma in adults. In: Tintinalli JE, Ma OJ, Yealy DM, Meckler GD, Stapczynski JS, Cline DM, Thomas SH, eds. Tintinalli’s Emergency Medicine: A Comprehensive Study Guide, 9e. New York: McGraw Hill; 2010. pp. 1669-76.

Doğukan M, Güler F, Baysal A, Güven C, Uludag Ö. Impact of tranexamic acid on bleeding during coronary artery bypass for patients under treatment of low molecular weight heparin. J Surg Med. 2020;4(4):309-13. DOI: https://doi.org/10.28982/josam.713471

Chiara O, Cimbanassi S, Bellanova G, Chiarugi M, Mingoli A, Olivero G, et al. A systematic review on the use of topical hemostats in trauma and emergency surgery. BMC Surg. 2018;18:68. DOI: https://doi.org/10.1186/s12893-018-0398-z

Abacıoğlu S, Aydın K, Büyükcam F, Kaya U, Işık B, Karakılıç ME. Comparison of the Efficiencies of Buffers Containing Ankaferd and Chitosan on Hemostasis in an Experimental Rat Model with Femoral Artery Bleeding. Turk J Haematol. 2016;33:48-52. DOI: https://doi.org/10.4274/tjh.2014.0029

Bjorenson JE, Grove HF, List Sr MG, Haasch GC, Austin BP. Effects of hemostatic agents on the pH of body fluids. J Endod. 1986;12:289-92. DOI: https://doi.org/10.1016/S0099-2399(86)80110-8

Clé-Ovejero A, Valmaseda-Castellón E. Haemostatic agents in apical surgery. A systematic review. Med Oral Patol Oral Cir Bucal. 2016;21:652-7. DOI: https://doi.org/10.4317/medoral.21109

Hori H, Hattori S, Inouye S, Kimura A, Irie S, Miyazawa H, et al. Analysis of the major epitope of the alpha2 chain of bovine type I collagen in children with bovine gelatin allergy. J Allergy Clin Immunol. 2002;110:652-7. DOI: https://doi.org/10.1067/mai.2002.127862

Shilo S, Roth S, Amzel T, Harel-Adar T, Tamir E, Grynspan F, et al. Cutaneous wound healing after treatment with plant-derived human recombinant collagen flowable gel. Tissue Eng Part A. 2013;19:1519-26. DOI: https://doi.org/10.1089/ten.tea.2012.0345

Tomizawa Y. Clinical benefits and risk analysis of topical hemostats: a review. J Artif Organs. 2005;8:137-42. DOI: https://doi.org/10.1007/s10047-005-0296-x

Punyanitya S, Thiansem S, Raksujarit A, Sontichai W, Koonawoot R. Rice Starch–Based Sponge for Use as Topical Hemostatic Agent. Key Eng Mater. 2019;803:153-7. DOI: https://doi.org/10.4028/www.scientific.net/KEM.803.153

Ruksanti A, Mahapram B, Thiansem S, Koonawoot R, Punyanitya S. Preparation and some Physical Characterization of Rice Starch-Carboxymethyl Cellulose as Hemostatic Film. Materials Science Forum. 2021;1042:117-22. DOI: https://doi.org/10.4028/www.scientific.net/MSF.1042.117

Sener D, Kocak M, Saracoglu R, Deveci U, Karadag M. Histopathological effects of Algan hemostatic agent (AHA) in liver injury model in rats. Hepatology Forum. 2022;1(3):16-20. DOI: https://doi.org/10.14744/hf.2021.2021.0040

Midi A, Ekici H, Kumandas A, Durmus O, Bodic B, Tiryaki M, et al. Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats. Marmara Med J. 2019;32:27-32. DOI: https://doi.org/10.5472/marumj.518821

Midi A, Kumandaş A, Ekici H, Arda S, Karahan S, Şimşek AK, et al. Investigation of the effectiveness of algan hemostatic agent in renal venous bleeding model in rats. EJMI. 2018;2:129–32. DOI: https://doi.org/10.14744/ejmi.2018.32032

Midi A, Kumandas A, Ekici H, Bayraktar F, Karapirli K, Karahan S, et al. Investigation of the efficacy of Algan Hemostatic Agent in liver laceration model in rats. EJMO. 2019;3:37-42. DOI: https://doi.org/10.14744/ejmo.2018.0008

Midi A, Ozyurek HE, Karahan S, Ekici H, Kumandas A, Turkmen I, et al. Investigation of efficacy of the plant based algan hemostatic agent in hepatectomy bleeding model in rats. EJMI. 2018;2:195-201. DOI: https://doi.org/10.14744/ejmi.2018.35744

Sogut O, Erdogan MO, Kose R, Boleken ME, Kaya H, Gokdemir MT, et al. Hemostatic Efficacy of a Traditional Medicinal Plant Extract (Ankaferd Blood Stopper) in Bleeding Control. Clinical and Applied Thrombosis/Hemostasis. 2015;21:348-53. DOI: https://doi.org/10.1177/1076029613504129

Chan MW, Schwaitzberg SD, Demcheva M, Vournakis J, Finkielsztein S, Connolly RJ. Comparison of poly-N-acetyl glucosamine (P-GlcNAc) with absorbable collagen (Actifoam), and fibrin sealant (Bolheal) for achieving hemostasis in a swine model of splenic hemorrhage. J Trauma. 2000;48:454-8. DOI: https://doi.org/10.1097/00005373-200003000-00013

Msezane LP, Katz MH, Gofrit ON, Shalhav AL, Zorn KC. Hemostatic agents and instruments in laparoscopic renal surgery. J Endourol. 2008;22:403-8. DOI: https://doi.org/10.1089/end.2007.9844

Wagner WR, Pachence JM, Ristich J, Johnson PC. Comparative in vitro analysis of topical hemostatic agents. J Surg Res. 1996;66:100-8. DOI: https://doi.org/10.1006/jsre.1996.0379

Hanks JB, Kjaergard HK, Hollingsbee DA. A comparison of the haemostatic effect of Vivostat patient-derived fibrin sealant with oxidised cellulose (Surgicel) in multiple surgical procedures. Eur Surg Res. 2003;35:439-44. DOI: https://doi.org/10.1159/000072229

Ersoy G, Kaynak MF, Yilmaz O, Rodoplu U, Maltepe F, Gokmen N. Hemostatic effects of microporous polysaccharide hemosphere in a rat model with severe femoral artery bleeding. Adv Ther. 2007;24:485-92. DOI: https://doi.org/10.1007/BF02848770

Wang YW, Liu CC, Cherng JH, Lin CS, Chang SJ, Hong ZJ, et al. Biological effects of chitosan-based dressing on hemostasis mechanism. Polymers. 2019;11(11):1906. DOI: https://doi.org/10.3390/polym11111906

Köksal Ö, Özdemir F, Etöz BC, Büyükcoşkun NI, Sığırlı D. Hemostatic effect of a chitosan linear polymer (Celox®) in a severe femoral artery bleeding rat model under hypothermia or warfarin therapy. Ulus Travma Acil Cerrahi Derg. 2011;17(3):199-204. DOI: https://doi.org/10.5505/tjtes.2011.88155

Bertram JP, Williams CA, Robinson R, Segal SS, Flynn NT, Lavik EB. Synthetic platelets: nanotechnology to halt bleeding. Sci Transl Med. 2009;1(11):11-22. DOI: https://doi.org/10.1126/scitranslmed.3000397

Heinen A, Welke V, Behmenburg F, Stroethoff M, Stoldt V, Hoffmann T, et al. Haemotherapy with Fibrinogen for Perioperative Bleeding Prevention-A View on Arterial Thrombogenesis and Myocardial Infarction in the Rat In Vivo. J Clin Med. 2019;8(6):880. DOI: https://doi.org/10.3390/jcm8060880

Chan KYT, Yong ASM, Wang X, Ringgold KM, St John AE, Baylis JR, et al. The adhesion of clots in wounds contributes to hemostasis and can be enhanced by coagulation factor XIII. Sci Rep. 2020;10(1):1-10. DOI: https://doi.org/10.1038/s41598-020-76782-z

Gedar Totuk ÖM, Güzel ŞE, Ekici H, Kumandaş A, Emre Aydıngöz S, Yılmaz EÇ, et al. Effects of Algan Hemostatic Agent on bleeding time in a rat tail hemorrhage model. Ulus Travma Acil Cerrahi Derg. 2020;26(6):853-8.

Aksoy H, Sener A, Akakin D, Şen A, Akpınar ÖB, Özcan GS, et al. The Effect of algan hemostatic agent (AHA) on wound healing. Clin Exp Health Sci. 2020;10(3):279-84. DOI: https://doi.org/10.33808/marusbed.767312

Downloads

Published

2022-07-29

Issue

Section

Research Article

How to Cite

1.
Şener D, Aydın A, Cücü E, Adademir T, Türet DM, Karadağ M. Effects of Algan hemostatic agent foam in rat femoral artery injury model: A randomized animal trial : Algan hemostatic agent act on artery injury model. J Surg Med [Internet]. 2022 Jul. 29 [cited 2022 Aug. 8];6(7):689-92. Available from: https://jsurgmed.com/article/view/1017655