Yıl 2021, Cilt 5 , Sayı 1, Sayfalar 26 - 30 2021-01-01

Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation

Ferda KESKİN ÇİMEN [1] , Orhan ÇİMEN [2] , Durdu ALTUNER [3] , Arif Burak ÇEKİÇ [4] , Nezahat KURT [5] , Halis SÜLEYMAN [6]


Background/Aim: The large amount of oxygen presented to the ischemic tissue in reperfusion causes the formation of excess free oxygen radicals and results in oxidative damage. Rutin is a flavonoid with potent antioxidant and anti-inflammatory effects. The aim of this study is to examine the effect of rutin on I/R-induced small intestinal (ileum) oxidative damage in rats. Methods: The animals were divided into three groups as follows: Intestinal ischemia-reperfusion (IIR), 50 mg/kg rutin+intestinal ischemia reperfusion (RIIR) and sham operation (Sham). Rutin was administered at a dose of 50 mg/kg by oral catheterization one hour prior to thiopental sodium anesthesia. Distilled water was administered with the same method to IIR and Sham groups as a solvent. To induce intestinal ischemia in RIIR and IIR groups, the superior mesenteric artery was suspended from the point where it left the aorta, and ischemia was induced for 45 minutes with the help of an atraumatic microvascular clamp followed by 60 minutes of reperfusion. Biochemical and histopathological examinations were performed on the dissected ileal tissues. Results: The amount of MDA and MPO activity increased, while tGSH levels and CAT activity decreased significantly in the intestinal tissue of the IIR group compared to sham group (P<0.001). Rutin treatment decreased the increase in MDA and MPO activity and increased the decrease of tGSH levels and CAT activity significantly compared to the IIR group (P<0.001). Histopathological changes such as PNL infiltration, edema, hemorrhage, and destruction were observed in the ileal tissue of the rats in the IIR group. However, there were no pathological findings in the RIIR group treated with rutin except for mildly dilated congested blood vessels. Conclusion: Rutin may be useful against intestinal I/R oxidative damage in clinical practice.
Rutin, small intestine, ischemia, reperfusion, rat
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Konular Patoloji
Bölüm Araştırma makalesi
Yazarlar

Orcid: 0000-0002-1844-0827
Yazar: Ferda KESKİN ÇİMEN
Kurum: ERZİNCAN BİNALİ YILDIRIM ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0003-4723-5838
Yazar: Orhan ÇİMEN
Kurum: ERZİNCAN BİNALİ YILDIRIM ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-5756-3459
Yazar: Durdu ALTUNER
Kurum: ERZİNCAN BİNALİ YILDIRIM ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-1460-6949
Yazar: Arif Burak ÇEKİÇ
Kurum: KARADENİZ TEKNİK ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-1685-5332
Yazar: Nezahat KURT
Kurum: ERZİNCAN BİNALİ YILDIRIM ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-9239-4099
Yazar: Halis SÜLEYMAN (Sorumlu Yazar)
Kurum: ERZİNCAN BİNALİ YILDIRIM ÜNİVERSİTESİ
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 1 Ocak 2021

Bibtex @araştırma makalesi { josam858237, journal = {Journal of Surgery and Medicine}, issn = {}, eissn = {2602-2079}, address = {jsurgmed@gmail.com}, publisher = {Fatih BAŞAK}, year = {2021}, volume = {5}, pages = {26 - 30}, doi = {10.28982/josam.858237}, title = {Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation}, key = {cite}, author = {Keskin Çimen, Ferda and Çimen, Orhan and Altuner, Durdu and Çekiç, Arif Burak and Kurt, Nezahat and Süleyman, Halis} }
APA Keskin Çimen, F , Çimen, O , Altuner, D , Çekiç, A , Kurt, N , Süleyman, H . (2021). Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation . Journal of Surgery and Medicine , 5 (1) , 26-30 . DOI: 10.28982/josam.858237
MLA Keskin Çimen, F , Çimen, O , Altuner, D , Çekiç, A , Kurt, N , Süleyman, H . "Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation" . Journal of Surgery and Medicine 5 (2021 ): 26-30 <http://jsurgmed.com/tr/pub/issue/59430/858237>
Chicago Keskin Çimen, F , Çimen, O , Altuner, D , Çekiç, A , Kurt, N , Süleyman, H . "Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation". Journal of Surgery and Medicine 5 (2021 ): 26-30
RIS TY - JOUR T1 - Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation AU - Ferda Keskin Çimen , Orhan Çimen , Durdu Altuner , Arif Burak Çekiç , Nezahat Kurt , Halis Süleyman Y1 - 2021 PY - 2021 N1 - doi: 10.28982/josam.858237 DO - 10.28982/josam.858237 T2 - Journal of Surgery and Medicine JF - Journal JO - JOR SP - 26 EP - 30 VL - 5 IS - 1 SN - -2602-2079 M3 - doi: 10.28982/josam.858237 UR - https://doi.org/10.28982/josam.858237 Y2 - 2021 ER -
EndNote %0 Journal of Surgery and Medicine Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation %A Ferda Keskin Çimen , Orhan Çimen , Durdu Altuner , Arif Burak Çekiç , Nezahat Kurt , Halis Süleyman %T Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation %D 2021 %J Journal of Surgery and Medicine %P -2602-2079 %V 5 %N 1 %R doi: 10.28982/josam.858237 %U 10.28982/josam.858237
ISNAD Keskin Çimen, Ferda , Çimen, Orhan , Altuner, Durdu , Çekiç, Arif Burak , Kurt, Nezahat , Süleyman, Halis . "Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation". Journal of Surgery and Medicine 5 / 1 (Ocak 2021): 26-30 . https://doi.org/10.28982/josam.858237
AMA Keskin Çimen F , Çimen O , Altuner D , Çekiç A , Kurt N , Süleyman H . Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation. J Surg Med. 2021; 5(1): 26-30.
Vancouver Keskin Çimen F , Çimen O , Altuner D , Çekiç A , Kurt N , Süleyman H . Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation. Journal of Surgery and Medicine. 2021; 5(1): 26-30.
IEEE F. Keskin Çimen , O. Çimen , D. Altuner , A. Çekiç , N. Kurt ve H. Süleyman , "Effect of rutin on experimentally induced small intestinal ischemia reperfusion injury in rats: A biochemical and histopathological evaluation", Journal of Surgery and Medicine, c. 5, sayı. 1, ss. 26-30, Oca. 2021, doi:10.28982/josam.858237