Year 2020, Volume 4 , Issue 6, Pages 451 - 455 2020-06-01

Streptozotosin ile diyabet oluşturulan ratlarda alt ekstremite iskemi reperfüzyonuna karşı fullerenol C60’ın etkileri
The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats

Hakan KARTAL [1] , Ayşegül KÜÇÜK [2] , Aydan KILIÇARSLAN [3] , Yücel POLAT [4] , Nuran SÜNGÜ [5] , Gülay KİP [6] , Mustafa ARSLAN [7]


Amaç: Suda çözünür bir fulleren olan Fullerenol C60'ın, serbest radikalleri in vitro ve in vivo temizleyebildiği gösterilmiştir. Çalışmanın amacı, fullerenol C60'ın iskemi reperfüzyon (I/R) sıçan modelinde alt iskelet kasları üzerindeki etkilerini araştırmaktı.
Yöntemler: Etik kurul onayı alındıktan sonra 30 Wistar Albino sıçan; 5 gruba ayrıldı (n: 6); Kontrol (C), diyabet (grup D), diyabet + fullerenol C60 grubu (DF), diyabet + I/R (grup DIR) ve diyabet I/R + fullerenol C60 (DIR-F). Diyabet için sıçanlara 55 mg / kg streptozotosin uygulandı. Diyabet oluşumundan dört hafta sonra sıçanlara 2 saatlik iskemi ve 2 saatlik reperfüzyon uygulandı. Reperfüzyon döneminin sonunda histopatolojik ve immünohistopatolojik incelemeler için tüm gruplardan alt ekstremite iskelet kası örnekleri alındı.
Bulgular: Miyozit ve endotelyal kaspaz 3 enzim aktiviteleri, özellikle DIR ve C, D, DF ve DIR-F grubunda yüksektir. Enflamasyon DIR grubunda C, DF ve DIR-F grubuna göre anlamlı olarak yüksektir (sırasıyla P<0,001, P=0,006, P<0,001). Myosit hasarı da DIR grubunda kontrol, C, DF ve DIR-F grubuna göre anlamlı derecede yüksektir (sırasıyla P<0,001, P=0,022, P<0,001). Vasküler dilatasyon ve konjesyon D, DF, DIR ve DIR-F grubunda kontrol grubuna göre anlamlı olarak yüksektir (P<0,001, tümü).
Sonuç: Sonuçlarımız, fullerenol C60'ın diyabetik sıçanlarda I/R'den kaynaklanan iskelet kası hasarına karşı koruyucu etkileri olduğunu doğrulamaktadır. Fullerenol C60'ın I/R hasarı üzerindeki etkilerini değerlendirmek için yapılacak gelecekteki çalışmalar, fullerenol C60'ın olası koruyucu etkilerini ve I/R hasarına bağlı doku hasarının altında yatan mekanizmaları anlamaya yardımcı olabilir.

Aim: Fullerenol, a water-soluble C60-fullerene, has been demonstrated to scavenge free radicals in vitro and in vivo. The aim of the study was to investigate the effects of fullerenol C60 on lower skeletal muscles in a rat model of ischemia/reperfusion (I/R).
Methods: After approval of the ethics committee, 30 Wistar Albino rat were divided into 5 groups with six animals per each as follows: Control (C), diabetes (D), diabetes+fullerenol C60 (DF), diabetes+I/R (group DIR) and diabetes I/R+fullerenol C60 (DIR-F) groups. Streptozotocin was administered to the rats to induce diabetes at a dose of 55 mg/kg. Four weeks after the onset of diabetes, rats were subjected to 2 hours of ischemia and 2 hours of reperfusion. At the end of the reperfusion period, skeletal muscle samples were taken from the lower extremity in all groups for histopathological and immunohistopathological examinations.
Results: Myositis and endothelial caspase 3 enzyme activities were high in all groups, particularly DIR. Compared to C, DF and DIR-F groups, inflammation and myositis were significantly higher in the DIR group (P=0.001, P=0.006, P=0.001, respectively, and P=0.001, P=0.022, P=0.001, respectively). Vascular dilatation and congestion were significantly more prominent in all groups compared to the control group (P=0.001 for all).
Conclusion: Our results confirm that fullerenol C60 has protective effects against skeletal muscle damage resulting from I/R in diabetic rats. Future studies conducted to evaluate these effects may help illuminate the action mechanism of fullerenol C60 and pathophysiology underlying the tissue damage related to I/R injury.
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Primary Language en
Subjects Cardiac and Cardiovascular Systems
Journal Section Research article
Authors

Orcid: 0000-0003-4539-0228
Author: Hakan KARTAL (Primary Author)
Institution: Department of Cardiovascular Surgery, Gulhane Medical Faculty, Gulhane Education and Research Hospital, Ankara-Turkey
Country: Turkey


Orcid: 0000-0001-9316-9574
Author: Ayşegül KÜÇÜK
Institution: KÜTAHYA SAĞLIK BİLİMLERİ ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0002-7981-4458
Author: Aydan KILIÇARSLAN
Institution: ANKARA YILDIRIM BEYAZIT UNIVERSITY
Country: Turkey


Orcid: 0000-0002-3733-7198
Author: Yücel POLAT
Institution: TEKİRDAĞ ŞARKÖY DEVLET HASTANESİ
Country: Turkey


Orcid: 0000-0001-5187-2616
Author: Nuran SÜNGÜ
Institution: ANKARA YILDIRIM BEYAZIT UNIVERSITY
Country: Turkey


Orcid: 0000-0001-5242-5332
Author: Gülay KİP
Institution: Gazı University
Country: Turkey


Orcid: 0000-0003-4882-5063
Author: Mustafa ARSLAN
Institution: Gazı University
Country: Turkey


Supporting Institution YOK
Dates

Publication Date : June 1, 2020

Bibtex @research article { josam756665, journal = {Journal of Surgery and Medicine}, issn = {}, eissn = {2602-2079}, address = {jsurgmed@gmail.com}, publisher = {Fatih BAŞAK}, year = {2020}, volume = {4}, pages = {451 - 455}, doi = {10.28982/josam.756665}, title = {The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats}, key = {cite}, author = {Kartal, Hakan and Küçük, Ayşegül and Kılıçarslan, Aydan and Polat, Yücel and Süngü, Nuran and Ki̇p, Gülay and Arslan, Mustafa} }
APA Kartal, H , Küçük, A , Kılıçarslan, A , Polat, Y , Süngü, N , Ki̇p, G , Arslan, M . (2020). The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats . Journal of Surgery and Medicine , 4 (6) , 451-455 . DOI: 10.28982/josam.756665
MLA Kartal, H , Küçük, A , Kılıçarslan, A , Polat, Y , Süngü, N , Ki̇p, G , Arslan, M . "The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats" . Journal of Surgery and Medicine 4 (2020 ): 451-455 <http://jsurgmed.com/en/pub/issue/54767/756665>
Chicago Kartal, H , Küçük, A , Kılıçarslan, A , Polat, Y , Süngü, N , Ki̇p, G , Arslan, M . "The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats". Journal of Surgery and Medicine 4 (2020 ): 451-455
RIS TY - JOUR T1 - The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats AU - Hakan Kartal , Ayşegül Küçük , Aydan Kılıçarslan , Yücel Polat , Nuran Süngü , Gülay Ki̇p , Mustafa Arslan Y1 - 2020 PY - 2020 N1 - doi: 10.28982/josam.756665 DO - 10.28982/josam.756665 T2 - Journal of Surgery and Medicine JF - Journal JO - JOR SP - 451 EP - 455 VL - 4 IS - 6 SN - -2602-2079 M3 - doi: 10.28982/josam.756665 UR - https://doi.org/10.28982/josam.756665 Y2 - 2020 ER -
EndNote %0 Journal of Surgery and Medicine The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats %A Hakan Kartal , Ayşegül Küçük , Aydan Kılıçarslan , Yücel Polat , Nuran Süngü , Gülay Ki̇p , Mustafa Arslan %T The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats %D 2020 %J Journal of Surgery and Medicine %P -2602-2079 %V 4 %N 6 %R doi: 10.28982/josam.756665 %U 10.28982/josam.756665
ISNAD Kartal, Hakan , Küçük, Ayşegül , Kılıçarslan, Aydan , Polat, Yücel , Süngü, Nuran , Ki̇p, Gülay , Arslan, Mustafa . "The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats". Journal of Surgery and Medicine 4 / 6 (June 2020): 451-455 . https://doi.org/10.28982/josam.756665
AMA Kartal H , Küçük A , Kılıçarslan A , Polat Y , Süngü N , Ki̇p G , Arslan M . The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats. J Surg Med. 2020; 4(6): 451-455.
Vancouver Kartal H , Küçük A , Kılıçarslan A , Polat Y , Süngü N , Ki̇p G , Arslan M . The effect of fullerenol C60 on skeletal muscle after lower limb ischemia reperfusion injury in streptozotocin-induced diabetic rats. Journal of Surgery and Medicine. 2020; 4(6): 451-455.