TY - JOUR AU - Eken, Huseyin AU - Kurnaz, Eray PY - 2019/07/29 Y2 - 2024/03/29 TI - Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury JF - Journal of Surgery and Medicine JA - J Surg Med VL - 3 IS - 7 SE - Research Article DO - 10.28982/josam.587598 UR - https://jsurgmed.com/article/view/587598 SP - 494-497 AB - <p><p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"><span style="font-size:12pt;font-family:'Times New Roman', serif;">Aim:Ischemia/reperfusion (I/R) procedure applied during liver resection andtransplantation in clinic settings causes liver oxidative damage. The aim ofthis study was to examine the effects of taxifolin on liver injury induced byI/R in rats. </span></p><p></p><p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"><span style="font-size:12pt;font-family:'Times New Roman', serif;">Methods: AlbinoWistar male rats divided into three groups with 6 rats in each group: liverischemia-reperfusion (LIR), 50 mg/kg taxifolin + liver ischemia reperfusion(TLIR) and sham operation (SHAM). An hour before thiopental sodium anesthesia,50 mg/kg taxifolin was orally administered to the TLIR group and distilledwater to the LIR and SHAM groups. In the TLIR and LIR groups, a clamp wasplaced in the hepatic artery, portal vein and bile duct, thereby inducingischemia for one hour, and reperfusion for six hours. In the SHAM group, theabdominal cavity was closed by surgical suture without any procedure. At theend of this period, rats were sacrificed with high dose anesthesia. Livertissues were removed for biochemical and histopathological examinations</span></p><p></p><p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"><span style="font-size:12pt;font-family:'Times New Roman', serif;"> Results: I/R procedure significantly increasedMDA (<i>P&lt;</i>0.001), ALT, AST levels (<i>P&lt;</i>0.001) and decreased tGSHlevel (<i>P&lt;</i>0.001) in liver tissue compared to SHAM group. In the taxifolintreated group, this effect was suppressed. Histopathologically, in the I/Rinduced group, pathological findings such as dilated congested blood vessel,hemorrhage, destruction in the liver parenchyma and edema observed. Livertissue in the taxifolin group had near-normal appearance except mild sinusoidalcongestion.</span></p><p></p><p><span style="font-size:12pt;line-height:115%;font-family:'Times New Roman', serif;">Conclusion: Our resultsindicate that taxifolin is an effective agent in reducing hepatic damage causedby I/R.</span><br /></p></p> ER -