Year 2019, Volume 3 , Issue 11, Pages 805 - 808 2019-11-01

Kafein ve melatoninin sıçan fetus akciğer dokularındaki antioksidan enzimler üzerine koruyucu etkisi
The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues

Mustafa NİSARİ [1] , Seher YILMAZ [2] , Ayşe Yeşim GÖÇMEN [3] , Ersin KARATAŞ [4] , Özge AL [5]


Amaç: Hamilelik sırasında bilinçli olarak kullanılan veya bilinçsizce maruz kalınan nikotin, alkol, kafein ve türevler gibi teratojenik maddelerin embriyonal ve fetal dokulara doğrudan veya dolaylı olarak zarar vermesinin yanı sıra anneye de zarar verebilir. Melatoninin farklı organ ve dokularda doğrudan serbest radikal toplayıcısı ve dolaylı antioksidan etkileri olduğu gösterilmiştir. Bu çalışmada, fetus akciğer dokularında gebe sıçanlara uygulanan yüksek ve düşük dozdaki kafeinin etkisine karşı güçlü bir antioksidan olan melatoninin değerlendirilmesi amaçlanmıştır.

Yöntemler: Çalışmamızda 35 adet yetişkin dişi Sprague-Dawley sıçan kullanıldı. Gebe sıçanlar, her birinde 5 sıçan olacak şekilde 7 gruba ayrıldı. Gebelere 20 gün boyunca invaziv işlemler uygulandı. Deney grubuna kafein, kafein yanı sıra tedavi gruplarına melatonin uygulandı. Yavrular sakrifiye edildi ve akciğer dokuları çıkarıldı. Süperoksit dismutaz (SOD), Glutatyon (GSH), Glutatyon disülfür (GSSG) toplam oksidan durumu (TOS), toplam antioksidan durumu (TAS), Kalsiyum (Ca) ve D vitamini (Vit D)) spektrofotometrik analiz ile ölçüldü. Oksidatif stres indeksi (OSI) ve Total glutation (GSH/ GSSG), oksidatif stres dokularının yararlı göstergeleridir ve sırasıyla TOS/TAS ve GSH/ GSSG oranı olarak hesaplandı.

Bulgular: M grubunda elde edilen en yüksek TAS değeri, GSH ve GSH / GSSG kontrol grubunda en yüksek, GSSG ise HDC grubunda en yüksek değere sahipti. HDC grubunda SOD diğer gruplara göre en yüksek değere sahipti (P<0,05).

Sonuçlar: Bu verilere göre, gebelikte kullanılan kafeinin akciğer gelişimini geciktirdiği ve güçlü bir antioksidan olan melatonin gecikmeyi en aza indirdiği gözlendi.

Aim: Teratogenic substances such as nicotine, alcohol, caffeine, and derivatives, which pregnant women may get exposed to unconsciously or use consciously can harm the mother and directly or indirectly damage embryonal and fetal tissues. Melatonin has been shown to exert direct free radical trapping and indirect antioxidant effects in different organs and tissues. In this study, we aimed to biochemically evaluate the effects of melatonin, a powerful antioxidant battling the oxidative effects of high and low doses of caffeine administered to pregnant rats in fetal lung tissues. 

Methods: In our study, 35 pregnant adult female Sprague-Dawley rats were used. Pregnant rats were divided into 7 groups with 5 rats in each. Caffeine and melatonin were administered for 20 days during the pregnancy. The gestational period lasted 21 days in average. The offspring were sacrificed, and lung tissues were removed. Superoxide dismutase (SOD), glutathione (GSH), glutathione disulfide (GSSG), total oxidant status (TOS), total antioxidant status (TAS), calcium (Ca) and vitamin D (Vit D) were measured by spectrophotometric assay. The oxidative stress index (OSI) and total glutathione (GSH/GSSG) were determinants of oxidative stress and were calculated as TOS/TAS and GSH/GSSG ratios, respectively.

Results: The highest TAS value was obtained in the Melatonin group (M) group. GSH and GSH/GSSG was highest in the control group, whereas GSSG was the highest in the high-dose caffeine group (HDC) group. HDC group had the highest SOD value compared to the other groups (P<0.05).

Conclusions: According to these data, it was determined that caffeine used during pregnancy delayed the development of lung, and melatonin, which is a strong antioxidant, minimized the delay.


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Primary Language en
Subjects Biochemistry and Molecular Biology
Journal Section Research article
Authors

Orcid: 0000-0001-7469-8921
Author: Mustafa NİSARİ (Primary Author)
Country: Turkey


Orcid: 0000-0003-4551-995X
Author: Seher YILMAZ
Institution: Yozgat Bozok University

Orcid: 0000-0002-8511-639X
Author: Ayşe Yeşim GÖÇMEN
Institution: Yozgat Bozok University

Orcid: 0000-0001-6848-7618
Author: Ersin KARATAŞ
Institution: GEBZE TECHNICAL UNIVERSITY

Orcid: 0000-0001-5292-3593
Author: Özge AL

Dates

Publication Date : November 1, 2019

Bibtex @research article { josam598742, journal = {Journal of Surgery and Medicine}, issn = {}, eissn = {2602-2079}, address = {jsurgmed@gmail.com}, publisher = {Fatih BAŞAK}, year = {2019}, volume = {3}, pages = {805 - 808}, doi = {10.28982/josam.598742}, title = {The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues}, key = {cite}, author = {Ni̇sari̇, Mustafa and Yılmaz, Seher and Göçmen, Ayşe Yeşim and Karataş, Ersin and Al, Özge} }
APA Ni̇sari̇, M , Yılmaz, S , Göçmen, A , Karataş, E , Al, Ö . (2019). The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues . Journal of Surgery and Medicine , 3 (11) , 805-808 . DOI: 10.28982/josam.598742
MLA Ni̇sari̇, M , Yılmaz, S , Göçmen, A , Karataş, E , Al, Ö . "The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues" . Journal of Surgery and Medicine 3 (2019 ): 805-808 <http://jsurgmed.com/en/pub/issue/49829/598742>
Chicago Ni̇sari̇, M , Yılmaz, S , Göçmen, A , Karataş, E , Al, Ö . "The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues". Journal of Surgery and Medicine 3 (2019 ): 805-808
RIS TY - JOUR T1 - The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues AU - Mustafa Ni̇sari̇ , Seher Yılmaz , Ayşe Yeşim Göçmen , Ersin Karataş , Özge Al Y1 - 2019 PY - 2019 N1 - doi: 10.28982/josam.598742 DO - 10.28982/josam.598742 T2 - Journal of Surgery and Medicine JF - Journal JO - JOR SP - 805 EP - 808 VL - 3 IS - 11 SN - -2602-2079 M3 - doi: 10.28982/josam.598742 UR - https://doi.org/10.28982/josam.598742 Y2 - 2019 ER -
EndNote %0 Journal of Surgery and Medicine The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues %A Mustafa Ni̇sari̇ , Seher Yılmaz , Ayşe Yeşim Göçmen , Ersin Karataş , Özge Al %T The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues %D 2019 %J Journal of Surgery and Medicine %P -2602-2079 %V 3 %N 11 %R doi: 10.28982/josam.598742 %U 10.28982/josam.598742
ISNAD Ni̇sari̇, Mustafa , Yılmaz, Seher , Göçmen, Ayşe Yeşim , Karataş, Ersin , Al, Özge . "The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues". Journal of Surgery and Medicine 3 / 11 (November 2019): 805-808 . https://doi.org/10.28982/josam.598742
AMA Ni̇sari̇ M , Yılmaz S , Göçmen A , Karataş E , Al Ö . The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues. J Surg Med. 2019; 3(11): 805-808.
Vancouver Ni̇sari̇ M , Yılmaz S , Göçmen A , Karataş E , Al Ö . The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues. Journal of Surgery and Medicine. 2019; 3(11): 805-808.
IEEE M. Ni̇sari̇ , S. Yılmaz , A. Göçmen , E. Karataş and Ö. Al , "The protective effects of caffeine and melatonin on antioxidant enzymes in rat fetal lung tissues", Journal of Surgery and Medicine, vol. 3, no. 11, pp. 805-808, Nov. 2019, doi:10.28982/josam.598742